Abstract
In this chapter we describe a method to generate mutants of filamentous fungi using their genomic plasticity and rapid adaptability to their environment. This method is based on spontaneous mutations occurring in relation to improved growth of fungi on media by repeated inoculation resulting in adaptation of the strain to the condition. The critical aspect of this method is the design of the selective media, which will depend strongly on the phenomenon that will be studied. This method is advantageous over UV or chemical random mutagenesis as it results in a lower frequency of undesired mutations and can result in strains that combined with (post)genomic approaches can enhance our understanding of the mechanisms driving various biological processes. In addition, it can be used to obtain better strains for various industrial applications. The method described here is specific for sporulating fungi and has so far not yet been tested for nonsporulating fungi.
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References
Gonzalez-Ramos D, Gorter de Vries AR, Grijseels SS, van Berkum MC, Swinnen S, van den Broek M, Nevoigt E, Daran JM, Pronk JT, van Maris AJ (2016) A new laboratory evolution approach to select for constitutive acetic acid tolerance in Saccharomyces cerevisiae and identification of causal mutations. Biotechnol Biofuels 9:173
Wisselink HW, Toirkens MJ, Wu Q, Pronk JT, van Maris AJ (2009) Novel evolutionary engineering approach for accelerated utilization of glucose, xylose, and arabinose mixtures by engineered Saccharomyces cerevisiae strains. Appl Environ Microbiol 75:907–914
Schoustra S, Punzalan D (2012) Correlation of mycelial growth rate with other phenotypic characters in evolved genotypes of Aspergillus nidulans. Fungal Biol 116:630–636
Schoustra SE, Bataillon T, Gifford DR, Kassen R (2009) The properties of adaptive walks in evolving populations of fungus. PLoS Biol 7:e1000250
Schoustra SE, Punzalan D, Dali R, Rundle HD, Kassen R (2012) Multivariate phenotypic divergence due to the fixation of beneficial mutations in experimentally evolved lineages of a filamentous fungus. PLoS One 7:e50305
Patyshakuliyeva A, Arentshorst M, Allijn IE, Ram AFJ, de Vries RP, Gelber IB (2016) Improving cellulase production by Aspergillus niger using adaptive evolution. Biotechnol Lett 38:969–974
Culleton H, Majoor E, McKie VA, de Vries RP (2016) Evolutionary adaptation as a tool to generate targeted mutant strains as evidence by increased inulinase production in Aspergillus oryzae. In: de Vries RP, Benoit Gelber I, Andersen MR (eds) Aspergillus and Penicillium in the post-genomic era. Caister Academic Press, Norfolk, UK, pp 189–196
Baracho MS, Baracho IR (2003) An analysis of the spontaneous mutation rate measurement in filamentous fungi. Genet Mol Biol 26:83–87
Lamb BC, Mandaokar S, Bahsoun B, Grishkan I, Nevo E (2008) Differences in spontaneous mutation frequencies as a function of environmental stress in soil fungi at “Evolution Canyon,” Israel. Proc Natl Acad Sci U S A 105:5792–5796
de Vries RP, Burgers K, van de Vondervoort PJI, Frisvad JC, Samson RA, Visser J (2004) A new black Aspergillus species, A. vadensis, is a promising host for homologous and heterologous protein production. Appl Environ Microbiol 70:3954–3959
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de Vries, R.P., Lubbers, R., Patyshakuliyeva, A., Wiebenga, A., Benoit-Gelber, I. (2018). Evolutionary Adaptation to Generate Mutants. In: de Vries, R., Tsang, A., Grigoriev, I. (eds) Fungal Genomics. Methods in Molecular Biology, vol 1775. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7804-5_12
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DOI: https://doi.org/10.1007/978-1-4939-7804-5_12
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