Abstract
Metabolomics based on mass spectrometry can provide quantitative and qualitative information of the pool of metabolites (metabolome) present intracellularly or extracellularly in a given biological system. A typical metabolomics workflow requires several key steps such as quick and robust sample preparations with quenching of metabolism, chemical derivatization if needed, instrumental measurement, data-processing with/without database information and further statistical analysis and interpretation. Here, we introduce general metabolomics workflows for global and targeted analyses using gas chromatography or liquid chromatography coupled with mass spectrometers.
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Acknowledgment
This chapter is based upon work supported by the US Department of Energy, Office of Science, Office of Biological and Environmental Research, Genomic Science program, under Award Number DE- SC0008744. This is also enabled by the Environmental Molecular Sciences Laboratory (EMSL), a national scientific user facility sponsored by the US Department of Energy’s (DOE) Office of Biological and Environmental Research (OBER), and located at the Pacific Northwest National Laboratory (PNNL). PNNL is a multiprogram national laboratory operated by Battelle for the DOE under Contract DE-AC05-76RLO 1830.
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Kim, YM., Heyman, H.M. (2018). Mass Spectrometry-Based Metabolomics. In: de Vries, R., Tsang, A., Grigoriev, I. (eds) Fungal Genomics. Methods in Molecular Biology, vol 1775. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7804-5_10
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DOI: https://doi.org/10.1007/978-1-4939-7804-5_10
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