Abstract
High-quality large molecular weight genomic DNA is important for genomic studies. Most commercial available genomic DNA purification kits have failed to generate high molecular weight DNA of sufficient quality from planarians. Here, we describe a simple and efficient genomic DNA isolation method, which has worked for several different planarian species, including Schmidtea mediterranea. This phenol–chloroform based method can be used to obtain genomic DNA of up to 150 kb and can be used for bacterial artificial chromosome (BAC) library construction, next-generation sequencing and PCR cloning.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Gregory SG, Sekhon M, Schein J, Zhao S, Osoegawa K, Scott CE et al (2002) A physical map of the mouse genome. Nature 418:743–750
Osoegawa K, de Jong PJ (2004) BAC library construction. In: Zhao S, Stodolsky M (eds) Bacterial artificial chromosomes volume 1: library construction, physical mapping, and sequencing, 1st edn. Humana Press, Totowa, NJ, p 1
Li R, Fan W, Tian G, Zhu H, He L, Cai J et al (2010) The sequence and de novo assembly of the giant panda genome. Nature 463:311–317
Wang K, Wang Z, Li F, Ye W, Wang J, Song G et al (2012) The draft genome of a diploid cotton Gossypium raimondii. Nat Genet 44:1098–1103
Huddleston J, Ranade S, Malig M, Antonacci F, Chaisson M, Hon L et al (2014) Reconstructing complex regions of genomes using long-read sequencing technology. Genome Res 24:688–696
Voskoboynik A, Neff NF, Sahoo D, Newman AM, Pushkarev D, Koh W et al (2013) The genome sequence of the colonial chordate, Botryllus schlosseri. Elife 2:e00569
Bowen ID, Ryder TA, Winters C (1975) The distribution of oxidizable mucosubstances and polysaccharides in the planarian Polycelis tenuis Iijima. Cell Tissue Res 161:263–275
Cantarel BL, Korf I, Robb SM, Parra G, Ross E, Moore B et al (2008) MAKER: an easy-to-use annotation pipeline designed for emerging model organism genomes. Genome Res 18:188–196
Garcia-Fernandez J, Baguna J, Salo E (1993) Genomic organization and expression of the planarian homeobox genes Dth-1 and Dth-2. Development 118:241–253
Cebria F, Newmark PA (2005) Planarian homologs of netrin and netrin receptor are required for proper regeneration of the central nervous system and the maintenance of nervous system architecture. Development 132:3691–3703
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Zhang, S., Alvarado, A.S. (2018). Planarian High Molecular Weight DNA Isolation by Spooling. In: Rink, J. (eds) Planarian Regeneration. Methods in Molecular Biology, vol 1774. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7802-1_8
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7802-1_8
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7800-7
Online ISBN: 978-1-4939-7802-1
eBook Packages: Springer Protocols