Abstract
High-quality genomic DNA extraction is a starting point for many downstream applications in modern molecular biology. Here, we describe a simple method for isolating high molecular weight genomic DNA from planarians. The method is based on tissue lysis by a mixture of a chaotropic salt and detergent followed by organic extraction to remove proteins and lipids followed by a postpurification step to remove contaminating polysaccharides. The isolated DNA is of high molecular weight and compatible with polymerase chain reaction, cloning, or next-generation sequencing library preparation.
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Acknowledgment
We would like to thank Sylke Winkler of the DNA sequencing core facility at the MPI-CBG for assistance with DNA quality control and quantification.
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Grohme, M.A., Vila-Farré, M., Rink, J.C. (2018). Small- and Large-Scale High Molecular Weight Genomic DNA Extraction from Planarians. In: Rink, J. (eds) Planarian Regeneration. Methods in Molecular Biology, vol 1774. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7802-1_7
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DOI: https://doi.org/10.1007/978-1-4939-7802-1_7
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7800-7
Online ISBN: 978-1-4939-7802-1
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