Correction to: A Subtractive FACS Method for Isolation of Planarian Stem Cells and Neural Cells

Correction to: Chapter 19 in: Jochen C. Rink (ed.), Planarian Regeneration: Methods and Protocols, Methods in Molecular Biology, vol. 1774, https://doi.org/10.1007/978-1-4939-7802-1_19

The original version of this chapter was inadvertently published without the below mentioned code listings in Chapter 19.

3.3. Cell Staining for Stem Cell Isolation

• 1. Add 18 μL of Hoechst 33342 stock solution and 0.5 μL of calcein AM stock solution to the filtered samples (Subheading 3.1, step 8) (see Note 10).

• 2. Invert the sample tubes about three times and incubate for 120 min at 20 °C (see Note 11).

• 3. Pellet by centrifuging at 1500 × g for 5 min and remove the supernatants. Resuspend cell pellets with 2 mL (two sample volumes) of 5/8 Holtfreter’s.

• 4. Add PI stock solution at 1 μL/mL to the cell suspensions and incubate at least 5 min on ice.

• 5. Proceed to FACS analysis or microscopy.

The code listings have been updated in the same chapter.