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Planarian Regeneration pp 379–392Cite as

Whole-Mount In Situ Hybridization of Planarians

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1774))

Abstract

Whole-mount in situ hybridization (WISH) and fluorescent whole-mount in situ hybridization (FISH) allow for visualization of specific mRNA transcripts to answer diverse biological questions. In planarians, in situ hybridization enables determination of gene expression profiles and identification of cell-type specific markers for analyzing experimental treatments. Here, we describe a robust whole-mount protocol for detecting gene expression patterns in the planarian Schmidtea mediterranea.

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Acknowledgments

We thank Amanda Adams, Caitlin Dingwall, Melanie Issigonis, and Rachel Roberts-Galbraith for comments on the manuscript and recommendations for processing sexual planarians.

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Authors and Affiliations

  1. St. Norbert College, De Pere, WI, USA

    Ryan S. King

  2. Department of Cell and Developmental Biology, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Ryan S. King & Phillip A. Newmark

  3. Howard Hughes Medical Institute, University of Illinois at Urbana-Champaign, Urbana, IL, USA

    Ryan S. King & Phillip A. Newmark

  4. Howard Hughes Medical Institute, Morgridge Institute for Research, Madison, WI, USA

    Phillip A. Newmark

  5. Department of Integrative Biology, University of Wisconsin–Madison, Madison, WI, USA

    Phillip A. Newmark

Authors
  1. Ryan S. King
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  2. Phillip A. Newmark
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Corresponding author

Correspondence to Ryan S. King .

Editor information

Editors and Affiliations

  1. Max Planck Institute of Molecular Cell Biology and Genetics, Dresden, Germany

    Jochen C. Rink

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King, R.S., Newmark, P.A. (2018). Whole-Mount In Situ Hybridization of Planarians. In: Rink, J. (eds) Planarian Regeneration. Methods in Molecular Biology, vol 1774. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7802-1_12

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  • DOI: https://doi.org/10.1007/978-1-4939-7802-1_12

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7800-7

  • Online ISBN: 978-1-4939-7802-1

  • eBook Packages: Springer Protocols

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