Skip to main content

Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation

  • Protocol

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1773))

Abstract

The cat, as a species, is somewhat new to the field of adult multipotent stromal cells. Despite the relative phylogenetic distance between the domestic cat, Felis silvestris catus, and humans, they share some similar health challenges like diabetes, kidney disease and asthma. There is a plethora of current investigative efforts focused on adult adipose tissue-derived multipotent stromal cell (ASC) therapies to address these and other conditions. Given the small size of domestic cats, particular attention to optimize cell isolation from relatively little tissue is a necessary condition of feline ASC studies and therapies. Additionally, there are some unique features of culture conditions to test and confirm feline ASC plasticity. This chapter contains a few of the novel aspects of feline ASC isolation and culture.

This is a preview of subscription content, log in via an institution.

Buying options

Protocol
USD   49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD   199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Springer Nature is developing a new tool to find and evaluate Protocols. Learn more

References

  1. Zhang N, Dietrich MA, Lopez MJ (2014) Therapeutic doses of multipotent stromal cells from minimal adipose tissue. Stem Cell Rev 10:600–611

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  2. Kalaszczynska I, Ruminski S, Platek AE et al (2013) Substantial differences between human and ovine mesenchymal stem cells in response to osteogenic media: how to explain and how to manage? Biores Open Access 2:356–363

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  3. Quimby JM, Webb TL, Habenicht LM et al (2013) Safety and efficacy of intravenous infusion of allogeneic cryopreserved mesenchymal stem cells for treatment of chronic kidney disease in cats: results of three sequential pilot studies. Stem Cell Res Ther 4:48

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  4. Quimby JM, Webb TL, Randall E et al (2016) Assessment of intravenous adipose-derived allogeneic mesenchymal stem cells for the treatment of feline chronic kidney disease: a randomized, placebo-controlled clinical trial in eight cats. J Feline Med Surg 18(2):165–171

    Article  PubMed  Google Scholar 

  5. Trzil JE, Masseau I, Webb TL et al (2014) Long-term evaluation of mesenchymal stem cell therapy in a feline model of chronic allergic asthma. Clin Exp Allergy 44:1546–1557

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  6. Volarevic V, Arsenijevic N, Lukic ML et al (2011) Concise review: mesenchymal stem cell treatment of the complications of diabetes mellitus. Stem Cells 29:5–10

    Article  PubMed  CAS  Google Scholar 

  7. Gimble JM, Nuttall ME (2011) Adipose-derived stromal/stem cells (ASC) in regenerative medicine: pharmaceutical applications. Curr Pharm Des 17:332–339

    Article  PubMed  CAS  Google Scholar 

  8. Spencer ND, Chun R, Vidal MA et al (2012) In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells. Vet J 191:231–239

    Article  PubMed  Google Scholar 

  9. Quimby JM, Webb TL, Gibbons DS et al (2011) Evaluation of intrarenal mesenchymal stem cell injection for treatment of chronic kidney disease in cats: a pilot study. J Feline Med Surg 13:418–426

    Article  PubMed  Google Scholar 

Download references

Acknowledgments

The authors gratefully acknowledge funding by the Winn Feline Foundation. Many thanks to members of the Laboratory for Equine and Comparative Orthopedic Research at Louisiana State University, School of Veterinary Medicine for their assistance with the preparation of this work

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Mandi J. Lopez .

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Cite this protocol

Fargason, C., Zhang, N., Lopez, M.J. (2018). Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation. In: Bunnell, B.A., Gimble, J.M. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 1773. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7799-4_15

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7799-4_15

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7797-0

  • Online ISBN: 978-1-4939-7799-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics