Abstract
The cat, as a species, is somewhat new to the field of adult multipotent stromal cells. Despite the relative phylogenetic distance between the domestic cat, Felis silvestris catus, and humans, they share some similar health challenges like diabetes, kidney disease and asthma. There is a plethora of current investigative efforts focused on adult adipose tissue-derived multipotent stromal cell (ASC) therapies to address these and other conditions. Given the small size of domestic cats, particular attention to optimize cell isolation from relatively little tissue is a necessary condition of feline ASC studies and therapies. Additionally, there are some unique features of culture conditions to test and confirm feline ASC plasticity. This chapter contains a few of the novel aspects of feline ASC isolation and culture.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsReferences
Zhang N, Dietrich MA, Lopez MJ (2014) Therapeutic doses of multipotent stromal cells from minimal adipose tissue. Stem Cell Rev 10:600–611
Kalaszczynska I, Ruminski S, Platek AE et al (2013) Substantial differences between human and ovine mesenchymal stem cells in response to osteogenic media: how to explain and how to manage? Biores Open Access 2:356–363
Quimby JM, Webb TL, Habenicht LM et al (2013) Safety and efficacy of intravenous infusion of allogeneic cryopreserved mesenchymal stem cells for treatment of chronic kidney disease in cats: results of three sequential pilot studies. Stem Cell Res Ther 4:48
Quimby JM, Webb TL, Randall E et al (2016) Assessment of intravenous adipose-derived allogeneic mesenchymal stem cells for the treatment of feline chronic kidney disease: a randomized, placebo-controlled clinical trial in eight cats. J Feline Med Surg 18(2):165–171
Trzil JE, Masseau I, Webb TL et al (2014) Long-term evaluation of mesenchymal stem cell therapy in a feline model of chronic allergic asthma. Clin Exp Allergy 44:1546–1557
Volarevic V, Arsenijevic N, Lukic ML et al (2011) Concise review: mesenchymal stem cell treatment of the complications of diabetes mellitus. Stem Cells 29:5–10
Gimble JM, Nuttall ME (2011) Adipose-derived stromal/stem cells (ASC) in regenerative medicine: pharmaceutical applications. Curr Pharm Des 17:332–339
Spencer ND, Chun R, Vidal MA et al (2012) In vitro expansion and differentiation of fresh and revitalized adult canine bone marrow-derived and adipose tissue-derived stromal cells. Vet J 191:231–239
Quimby JM, Webb TL, Gibbons DS et al (2011) Evaluation of intrarenal mesenchymal stem cell injection for treatment of chronic kidney disease in cats: a pilot study. J Feline Med Surg 13:418–426
Acknowledgments
The authors gratefully acknowledge funding by the Winn Feline Foundation. Many thanks to members of the Laboratory for Equine and Comparative Orthopedic Research at Louisiana State University, School of Veterinary Medicine for their assistance with the preparation of this work
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Fargason, C., Zhang, N., Lopez, M.J. (2018). Feline Adult Adipose Tissue-Derived Multipotent Stromal Cell Isolation and Differentiation. In: Bunnell, B.A., Gimble, J.M. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 1773. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7799-4_15
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7799-4_15
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7797-0
Online ISBN: 978-1-4939-7799-4
eBook Packages: Springer Protocols