Abstract
Evidence from preclinical research and clinical trials demonstrates the use of the stromal vascular fraction (SVF) as therapy for numerous indications. These results demonstrate that autologous SVF is not only safe and effective but provides robust anti-inflammatory, immunomodulatory, and reparative effects in vivo. The potency of the SVF is attributed to the cellular composition which includes adipose-derived stem cells (ASCs), adipocytes, endothelial cells, and various immune cells. As the name would suggest, these SVF cells are derived from the stromal compartment of adipose, or fat. Once digested, the cells that constitute adipose are released and collected as the SVF. The cellular frequencies within the SVF can then be assessed using a fluorescent antibody-based technique known as flow cytometry. The following chapter provides a standard operating protocol that describes the procedures from harvesting the fat tissue from experimental mice to isolating and characterizing the SVF.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Fruhbeck G (2008) Overview of adipose tissue and its role in obesity and metabolic disorders. Methods Mol Biol 456:1–22
Coelho M, Oliveira T, Fernandes R (2013) Biochemistry of adipose tissue: an endocrine organ. Arch Med Sci 9:191–200
Boutens L, Stienstra R (2016) Adipose tissue macrophages: going off track during obesity. Diabetologia 59(5):879–894
Lin G, Garcia M, Ning H et al (2008) Defining stem and progenitor cells within adipose tissue. Stem Cells Dev 17:1053–1063
Gimble JM, Katz AJ, Bunnell BA (2007) Adipose-derived stem cells for regenerative medicine. Circ Res 100:1249–1260
Wu AY (2013) Autologous fat transfer with in-situ mediation (AIM): a novel and compliant method of adult mesenchymal stem cell therapy. J Transl Med 11:136
Leto Barone AA, Khalifian S, Lee WP et al (2013) Immunomodulatory effects of adipose-derived stem cells: fact or fiction? Biomed Res Int 2013:383685
Trojahn Kolle SF, Oliveri RS, Glovinski PV et al (2012) Importance of mesenchymal stem cells in autologous fat grafting: a systematic review of existing studies. J Plast Surg Hand Surg 46:59–68
Volk SW, Theoret C (2013) Translating stem cell therapies: the role of companion animals in regenerative medicine. Wound Repair Regen 21:382–394
Black LL, Gaynor J, Gahring D et al (2007) Effect of adipose-derived mesenchymal stem and regenerative cells on lameness in dogs with chronic osteoarthritis of the coxofemoral joints: a randomized, double-blinded, multicenter, controlled trial. Vet Ther 8:272–284
Semon JA, Zhang X, Pandey AC et al (2013) Administration of murine stromal vascular fraction ameliorates chronic experimental autoimmune encephalomyelitis. Stem Cells Transl Med 2:789–796
Bowles AC, Strong AL, Wise RM, Thomas RC, Gerstein BY, Dutreil MF, Hunter RS, Gimble JM, Bunnell BA (2017) Adipose stromal vascular fraction-mediated improvements at late-stage disease in a murine model of multiple sclerosis. STEM CELLS 35(2):532–544
Ludwig DB, Trotter JT, Gabrielson JP, Carpenter JF, Randolph TW (2011) Flow cytometry: A promising technique for the study of silicone oil-induced particulate formation in protein formulations. Anal Biochem 410(2):191–199
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Bowles, A.C., Tucker, A., Bunnell, B.A. (2018). Isolation and Flow Cytometric Analysis of the Stromal Vascular Fraction Isolated from Mouse Adipose Tissue. In: Bunnell, B.A., Gimble, J.M. (eds) Adipose-Derived Stem Cells. Methods in Molecular Biology, vol 1773. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7799-4_1
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7799-4_1
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7797-0
Online ISBN: 978-1-4939-7799-4
eBook Packages: Springer Protocols