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Rapid Control of Genome Editing in Human Cells by Chemical-Inducible CRISPR-Cas Systems

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Synthetic Biology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1772))

Abstract

Genome editing using programmable DNA endonucleases enables the engineering of eukaryotic cells and living organisms with desirable properties or traits. Among the various molecular scissors that have been developed to date, the most versatile and easy-to-use family of nucleases derives from CRISPR-Cas, which exists naturally as an adaptive immune system in bacteria. Recent advances in the CRISPR-Cas technology have expanded our ability to manipulate complex genomes for myriad biomedical and biotechnological applications. Some of these applications are time-sensitive or demand high spatial precision. Here, we describe the use of an inducible CRISPR-Cas9 system, termed iCas, which we have developed to enable rapid and tight control of genome editing in mammalian cells. The iCas system can be switched on or off as desired through the introduction or removal of the small molecule tamoxifen or its related analogs such as 4-hydroxytamoxifen (4-HT).

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Author information

Authors and Affiliations

  1. School of Chemical and Biomedical Engineering, Nanyang Technological University, Singapore, Singapore

    Meng How Tan

  2. Genome Institute of Singapore, Agency for Science Technology and Research, Singapore, Singapore

    Kaiwen Ivy Liu, Muhammad Nadzim Bin Ramli, Norfala-Aliah Binte Sutrisnoh & Meng How Tan

Authors
  1. Kaiwen Ivy Liu
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  2. Muhammad Nadzim Bin Ramli
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  3. Norfala-Aliah Binte Sutrisnoh
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  4. Meng How Tan
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Corresponding author

Correspondence to Meng How Tan .

Editor information

Editors and Affiliations

  1. Agilent Technologies, Inc, La Jolla, California, USA

    Jeffrey Carl Braman

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Liu, K.I., Ramli, M.N.B., Sutrisnoh, NA.B., Tan, M.H. (2018). Rapid Control of Genome Editing in Human Cells by Chemical-Inducible CRISPR-Cas Systems. In: Braman, J. (eds) Synthetic Biology. Methods in Molecular Biology, vol 1772. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7795-6_15

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  • DOI: https://doi.org/10.1007/978-1-4939-7795-6_15

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7794-9

  • Online ISBN: 978-1-4939-7795-6

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