Hydrogel Printing Based on UV-Induced Projection for Cell-Based Microarray Fabrication

  • Wenguang Yang
  • Haibo Yu
  • Yuechao Wang
  • Lianqing Liu
Part of the Methods in Molecular Biology book series (MIMB, volume 1771)


A considerable number of studies have focused on fabrication of hydrogel microstructures due to its wide applications in tissue engineering, drug delivery, and extracellular matrix construction. Here, we introduce a hydrogel printing method based on UV-induced projection via a digital micromirror device (DMD). Arbitrary microstructures could be fabricated within few seconds (<3) by modulating UV projection using DMD as digital dynamic masks instead of a physical mask, which also offers a high degree of flexibility and repeatability. Furthermore, the ability of PEGDA film to hinder cell adhesion makes it possible to control area over which cells attach.

Key words

Microfabrication Hydrogel Microwell Cell pattern 



This work was partially supported by the National Natural Science Foundation of China (Project Nos. 61302003, 61475183, and 61503258), the NSFC/RGC Joint Research Scheme (Project No. 51461165501), and the CAS FEA International Partnership Program for Creative Research Teams.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Wenguang Yang
    • 1
  • Haibo Yu
    • 1
  • Yuechao Wang
    • 1
  • Lianqing Liu
    • 1
  1. 1.State Key Laboratory of Robotics, Shenyang Institute of AutomationChinese Academy of SciencesShenyangChina

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