Abstract
Biomolecule microarrays are generally produced by conventional microarrayer, i.e., by contact or inkjet printing. Microcontact printing represents an alternative way of deposition of biomolecules on solid supports but even if various biomolecules have been successfully microcontact printed, the production of biomolecule microarrays in routine by microcontact printing remains a challenging task and needs an effective, fast, robust, and low-cost automation process. Here, we describe the production of biomolecule microarrays composed of extracellular matrix protein for the fabrication of cell microarrays by using an automated microcontact printing device. Large scale cell microarrays can be reproducibly obtained by this method.
Key words
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Ruiz SA, Chen CS (2007) Microcontact printing: a tool to pattern. Soft Matter 3:168–177
Lange SA, Benes V, Kern DP, Hörber H, Bernard A (2004) Microcontact printing of DNA molecules. Anal Chem 76:1641–1647
Thibault C, Le Berre V, Casimirius S, Trévisiol E, François JM, Vieu C (2005) Direct microcontact printing of oligonucleotides for biochip applications. J Nanobiotechnol 3:7
Fredonnet J, Foncy J, Cau JC, Séverac C, François JM, Trévisiol E (2016) Automated and multiplexed soft lithography for the production of low-density DNA microarrays. Microarrays 5:25
Voskuhl J, Brinkmann J, Jonkheijm P (2014) Advances in contact printing technologies of carbohydrate, peptide and protein arrays. Curr Opin Chem Biol 18:1–7
Bernard A, Delamarche E, Schmid H, Michel B, Bosshard HR, Biebuyck H (1998) Printing patterns of proteins. Langmuir 14:2225–2229
Renaud JP, Bernard A, Bietsch A, Michel B, Bosshard HR, Delamarche E (2003) Fabricating arrays of single protein molecules on glass using microcontact printing. J Phys Chem B 108:703–711
Ricoult SG, Nezhad AS, Knapp-Mohammady M, Kennedy TE, Juncker D (2014) Humidified microcontact printing of proteins: universal patterning of proteins on both low and high energy surfaces. Langmuir 30:12002–12010
Hartmann M, Roeraade J, Stoll D, Templin MF, Joos TO (2009) Protein microarrays for diagnostic assays. Anal Bioanal Chem 393:1407–1416
Sin MLY, Mach KE, Wong PK, Liao JC (2014) Advances and challenges in biosensor-based diagnosis of infectious diseases. Expert Rev Mol Diagn 14:225–244
Shen K, Thomas VK, Dustin ML, Kam LC (2008) Micropatterning of costimulatory ligands enhances CD4+ T cell function. Proc Natl Acad Sci U S A 105:7791–7796
Fritz M, Bastmeyer M (2013) Microcontact printing of substrate-bound protein patterns for cell and tissue culture. Methods Mol Biol 1018:247–259
McBeath R, Pirone DM, Nelson CM, Bhadriraju K, Chen CS (2004) Cell shape, cytoskeletal tension, and RhoA regulate stem cell lineage commitment. Dev Cell 6:483–495
Tseng Q, Duchemin-Pelletier E, Deshiere A, Balland M, Guillou H, Filhol O, Théry M (2012) Spatial organization of extracellular matrix regulates cell-cell junction positioning. Proc Natl Acad Sci U S A 109:1506–1511
Gao L, McBeath R, Chen CS (2010) Stem cell shape regulates a chondrogenic versus myogenic fate through Rac1 and N-cadherin. Stem Cells 28:564–572
Huang S, Ingber DE (2000) Shape-dependent control of cell growth, differentiation, and apoptosis: switching between attractors in cell regulatory networks. Exp Cell Res 25:91–103
Théry M, Racine V, Pépin A, Piel M, Chen Y, Sibarita JB, Bornens M (2005) The extracellular matrix guides the orientation of the cell division axis. Nat Cell Biol 7:947–953
Dupin I, Camand E, Etienne-Manneville S (2009) Classical cadherins control nucleus and centrosome position and cell polarity. J Cell Biol 185:779–786
Desai RA, Gao L, Raghavan S, Liu WF, Chen CS (2009) Cell polarity triggered by cell-cell adhesion via E-cadherin. J Cell Sci 122:905–911
Théry M, Bornens M (2006) Cell shape and cell division. Curr Opin Cell Biol 18:648–657
Parker KK, Brock AL, Brangwynne C, Mannix RJ, Wang N, Ostuni E, Geisse NA, Adams JC, Whitesides GM, Ingber DE (2002) Directional control of lamellipodia extension by constraining cell shape and orientating cell tractional forces. FASEB J 16(10):1195–1204
Kane RS, Takayama S, Ostuni E, Ingber DE, Whitesides GM (1999) Patterning proteins and cells using soft lithography. Biomaterials 20:2363–2376
Brock A, Chang E, Ho CC, LeDuc P, Jiang X, Whitesides GM, Ingber DE (2003) Geometric determinants of directional cell motility revealed using microcontact printing. Langmuir 19:1611–1617
Mrksich M, Dike LE, Tien J, Ingber DE, Whitesides GM (1997) Using microcontact printing to pattern the attachment of mammalian cells to self-assembled monolayers of alkanethiolates on transparent films of gold and silver. Exp Cell Res 15:305–313
Théry M (2010) Micropatterning as a tool to decipher cell morphogenesis and functions. J Cell Sci 123:4201–4213
James J, Goluch ED, Hu H, Mrksich M (2008) Subcellular curvature at the perimeter of micropatterned cells influences lamellipodial distribution and cell polarity. Cell Motil Cytoskeleton 65:841–852
Cau JC, Lafforgue L, Nogues M, Lagraulet A, Paveau V (2013) Magnetic field assisted microcontact printing: a new concept of fully automated and calibrated process. Microelectron Eng 110:207–214
Acknowledgments
This work was supported by the national research agency “LABCOM” program (ANR-13-LAB2-0009-01) and partly supported by LAAS CNRS micro and nanotechnology facilities platform (member of the French RENATECH network). We thank Charline Blatché for her assistance in the cell microarray fabrication.
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Foncy, J. et al. (2018). Fabrication of Biomolecule Microarrays for Cell Immobilization Using Automated Microcontact Printing. In: Ertl, P., Rothbauer, M. (eds) Cell-Based Microarrays. Methods in Molecular Biology, vol 1771. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7792-5_7
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7792-5_7
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7791-8
Online ISBN: 978-1-4939-7792-5
eBook Packages: Springer Protocols