Artificial Microniche Array with Spatially Structured Biochemical Cues
We present here a method to create arrays of microcavities that can be differentially coated on their bottom, side, and top with different proteins. These cavities range in size from single cell to multicellular aggregate. We provide detailed protocols to create such arrays with some variations using different materials and different coating proteins. The use of such cavities as bona fide artificial microniches to mimic cellular microenvironments has been already established and is referenced.
Key wordsArray Microcavities Artificial microniche Cellular microenvironment
The authors acknowledge financial support from the Mechanobiology Institute of Singapore and from CNRS (France).