Oxygen-Generating Photocrosslinkable Hydrogel
Providing sufficient amount of oxygen to the cells is a critical issue since the lack of adequate oxygen leads to cell death and tissue necrosis. Therefore, there is a vital need to design and fabricate oxygen-generating biomaterials to mitigate hypoxia-induced cell death in engineered tissues. Here, we report the fabrication of an oxygen-generating hydrogel by incorporating calcium peroxide (CPO) into the methacrylated gelatin (GelMA) structure using photocrosslinking process. A sustainable release of oxygen could be provided from CPO-GelMA hydrogel over a period of 5 days under hypoxic conditions (1% O2).
Key wordsGelatin Photocrosslinking Oxygen-generating hydrogel
The authors acknowledge funding from the National Science Foundation (EFRI-1240443), IMMODGEL (602694), and the National Institutes of Health (EB012597, AR057837, DE021468, HL099073, AI105024, and AR063745).
- 10.Alemdar N, Leijten J, Camci-Unal G, Hjortnaes J, Ribas J, Paul A, Mostafalu P, Gaharwar AK, Qiu Y, Sonkusale S, Liao R, Khademhosseini A (2016) Oxygen-generating photo-cross-linkable hydrogels support cardiac progenitor cell survival by reducing hypoxia-induced necrosis. ACS Biomater Sci Eng. https://doi.org/10.1021/acsbiomaterials.6b00109