Oxygen-Generating Photocrosslinkable Hydrogel

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1771)

Abstract

Providing sufficient amount of oxygen to the cells is a critical issue since the lack of adequate oxygen leads to cell death and tissue necrosis. Therefore, there is a vital need to design and fabricate oxygen-generating biomaterials to mitigate hypoxia-induced cell death in engineered tissues. Here, we report the fabrication of an oxygen-generating hydrogel by incorporating calcium peroxide (CPO) into the methacrylated gelatin (GelMA) structure using photocrosslinking process. A sustainable release of oxygen could be provided from CPO-GelMA hydrogel over a period of 5 days under hypoxic conditions (1% O2).

Key words

Gelatin Photocrosslinking Oxygen-generating hydrogel 

Notes

Acknowledgment

The authors acknowledge funding from the National Science Foundation (EFRI-1240443), IMMODGEL (602694), and the National Institutes of Health (EB012597, AR057837, DE021468, HL099073, AI105024, and AR063745).

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Chemical EngineeringMarmara UniversityIstanbulTurkey

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