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Cell-Based Microarrays pp 213–224Cite as

Establishment of Microfluidic Spheroid Cultures for Biomedical Applications

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1771))

Abstract

Multicellular spheroid is a three-dimensional (3D) cell culture model that mimics cancer tumor environment. Its widespread use for anticancer therapy evaluation is nowadays limited by accessibility of 3D compatible assays. Here, a microfluidic system for spheroid formation, culture and analysis is presented. The system is compatible with standard microplate readers. The microfluidic chip enables long-term 3D cell culture and in situ monitoring of cellular viability. Moreover, design of the assay enables observation of delayed type of toxicity or application of repeated doses of a drug.

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Author information

Authors and Affiliations

  1. Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland

    Karina Kwapiszewska

Authors
  1. Karina Kwapiszewska
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Editor information

Editors and Affiliations

  1. Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria

    Peter Ertl

  2. Faculty of Technical Chemistry, Institute of Applied Synthetic Chemistry, Vienna University of Technology, Vienna, Austria

    Mario Rothbauer

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Kwapiszewska, K. (2018). Establishment of Microfluidic Spheroid Cultures for Biomedical Applications. In: Ertl, P., Rothbauer, M. (eds) Cell-Based Microarrays. Methods in Molecular Biology, vol 1771. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7792-5_17

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  • DOI: https://doi.org/10.1007/978-1-4939-7792-5_17

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7791-8

  • Online ISBN: 978-1-4939-7792-5

  • eBook Packages: Springer Protocols

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