Abstract
In many biochip applications, it is advantageous to be able to immobilize biomolecules at specific locations on the surface of solid supports. In this protocol, we describe a photochemical surface patterning procedure based on thiol–ene/yne photochemistry which allows for the simple and rapid selective patterning of biomolecules on thiol–ene solid supports. We describe the preparation of solid supports which are required for the immobilization, including porous monoliths, as well as two different immobilization schemes based on biotin–streptavidin interactions and covalent linkage via free amino groups respectively.
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Jönsson, A., Lafleur, J.P. (2018). Fabrication of Biomolecule Microarrays Using Rapid Photochemical Surface Patterning in Thiol–Ene-Based Microfluidic Devices. In: Ertl, P., Rothbauer, M. (eds) Cell-Based Microarrays. Methods in Molecular Biology, vol 1771. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7792-5_14
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DOI: https://doi.org/10.1007/978-1-4939-7792-5_14
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7791-8
Online ISBN: 978-1-4939-7792-5
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