Fabrication of Multielectrode Arrays for Neurobiology Applications

  • Mario Malerba
  • Hayder Amin
  • Gian N. Angotzi
  • Alessandro Maccione
  • Luca Berdondini
Part of the Methods in Molecular Biology book series (MIMB, volume 1771)


Substrate-integrated multielectrode arrays (MEAs) enable multisite, long-term, and label-free sensing and actuation of neuronal electrical signals in reduced cell culture models for network electrophysiology. Conventional, thin-film fabricated passive MEAs typically provide a few tens of electrode sites. New generations of active CMOS-based high-resolution arrays provide the capabilities of simultaneous recordings from thousands of neurons over fields of view of several square millimeters, yet allowing extracellular electrical imaging to be achieved down to the subcellular scale. In turn, such advancement in chip-based electrical readouts can significantly complement recently developed biotechnological and bimolecular techniques for neurobiology applications. Here, we describe (1) a simple method to fabricate passive MEAs and (2) protocols for preparing and growing primary rat hippocampal neuronal cultures and human iPS-derived neurons on MEAs. The aim is to provide reliable protocols for initiating the reader to this technology and for stimulating their further development and experimental use in neurobiology.

Key words

Multielectrode arrays (MEAs) CMOS-MEAs Network electrophysiology Primary rat hippocampal neuronal cultures Human iPS-derived neuronal cultures 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Mario Malerba
    • 1
  • Hayder Amin
    • 1
  • Gian N. Angotzi
    • 1
  • Alessandro Maccione
    • 1
  • Luca Berdondini
    • 1
  1. 1.Fondazione Istituto Italiano di Tecnologia (IIT)Neuroscience and Brain TechnologiesGenoaItaly

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