Primary and Stem Cell Microarrays: Application as Miniaturized Biotesting Systems
The deposition of living cells on microarray surfaces can be used to create physiologically relevant architecture in vitro. Such living cell microarrays enable the reconstruction of biological processes outside the body in a miniaturized format and have many advantages over traditional cell culture. The present protocol offers an option for the preparation and analysis of living primary and stem cell-based microarrays utilizing the standard microarray equipment (contact-free piezoelectric nanoprinter, microarray scanner), as well as microscopy. To produce living cell microarrays, we applied two kinds of mesenchymal stem cells (MSCs) isolated from umbilical cord and adipose tissue, as well as human umbilical vein endothelial cells (HUVECs) as model cells. We used live imaging microscopy for the online monitoring of cell spots in total size, staining of viable cells with Calcein acetoxymethyl ester (Calcein-AM) and treatment of MSCs with differentiation media to analyze the proliferation, viability, and differentiation potential of printed cells. This way, the general applicability of the established living cell-based microarray production was demonstrated.
Key wordsMicroarray technology Piezoelectric nanoprinting Living cell microarrays Primary human cells Mesenchymal stem cells Endothelial cells Online monitoring microscopy
This work was supported by the BIOFABRICATION FOR NIFE Initiative, which is financially supported by the Lower Saxony ministry of Science and Culture and the VolkswagenStiftung. NIFE is the Lower Saxony Center for Biomedical Engineering, Implant Research and Development in Hannover, a joint translational research centre of the Hannover Medical School, the Leibniz Universität Hannover, the University of Veterinary Medicine Hannover, and the Laser Center Hannover.
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