Advertisement

Next-Generation Live-Cell Microarray Technologies

  • Mario Rothbauer
  • Peter Ertl
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1771)

Abstract

Over the last decades the application of cell-based assays and in vitro cell culture systems has fundamentally transformed our understanding of biological functions on a cellular and organism level. The resulting ubiquitous usage of cell-based assays in today’s scientific world has therefore generated a need for advanced in vitro diagnostic systems. This increased demand has further led to the development of miniaturized live-cell microarrays for biomedical applications including high-throughput screening tools and microfluidic systems. The greatest benefit of miniaturized cell analysis systems is the ability to provide quantitative data in real time with high reliability and sensitivity, which are key parameters for any cell-based assay. An additional advantage of live-cell microarrays is their inherent capability for large-scale screening of single cells, multicell populations, as well as spheroids.

Key words

Live-cell microarrays Cell-based assays In vitro diagnostics 

References

  1. 1.
    Wartmann D, Rothbauer M, Kuten O, Barresi C, Visus C, Felzmann T, Ertl P (2015) Automated, miniaturized, and integrated quality control-on-chip (QC-on-a-Chip) for cell-based cancer therapy applications. Frontiers in Materials 2(60).  https://doi.org/10.3389/fmats.2015.00060
  2. 2.
    Rothbauer M, Charwat V, Ertl P (2016) Cell microarrays for biomedical applications. Methods Mol Biol 1368:273–291.  https://doi.org/10.1007/978-1-4939-3136-1_19CrossRefPubMedPubMedCentralGoogle Scholar
  3. 3.
    Mahto SK, Charwat V, Ertl P, Rothen-Rutishauser B, Rhee SW, Sznitman J (2015) Microfluidic platforms for advanced risk assessments of nanomaterials. Nanotoxicology 9(3):381–395.  https://doi.org/10.3109/17435390.2014.940402CrossRefPubMedPubMedCentralGoogle Scholar
  4. 4.
    Rothbauer M, Wartmann D, Charwat V, Ertl P (2015) Recent advances and future applications of microfluidic live-cell microarrays. Biotechnol Adv 33(6 Pt 1):948–961.  https://doi.org/10.1016/j.biotechadv.2015.06.006CrossRefGoogle Scholar
  5. 5.
    Selimović Š, Dokmeci MR, Khademhosseini A (2013) Organs-on-a-chip for drug discovery. Curr Opin Pharmacol 13(5):829–833.  https://doi.org/10.1016/j.coph.2013.06.005CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Wegener J (2015) Cell-based microarrays for in vitro toxicology. Annu Rev Anal Chem (Palo Alto, Calif) 8:335–358.  https://doi.org/10.1146/annurev-anchem-071213-020051CrossRefGoogle Scholar
  7. 7.
    Rosser J, Olmos I, Schlager M, Purtscher M, Jenner F, Ertl P (2015) Recent advances of biologically inspired 3D microfluidic hydrogel cell culture systems. J Cell Biol Cell Metab 2:005Google Scholar
  8. 8.
    Charwat V, Rothbauer M, Tedde SF, Hayden O, Bosch JJ, Muellner P, Hainberger R, Ertl P (2013) Monitoring dynamic interactions of tumor cells with tissue and immune cells in a lab-on-a-chip. Anal Chem 85(23):11471–11478.  https://doi.org/10.1021/ac4033406CrossRefPubMedPubMedCentralGoogle Scholar
  9. 9.
    Mairhofer J, Roppert K, Ertl P (2009) Microfluidic systems for pathogen sensing: a review. Sensors (Basel) 9(6):4804–4823.  https://doi.org/10.3390/s90604804CrossRefGoogle Scholar
  10. 10.
    Rothbauer M, Kupcu S, Sleytr UB, Ertl P (2015) Crystalline bacterial protein Nanolayers for cell micropatterning. IFMBE Proc 45:337–340.  https://doi.org/10.1007/978-3-319-11128-5_84CrossRefGoogle Scholar
  11. 11.
    Rothbauer M, Ertl P, Theiler BA, Schlager M, Sleytr UB, Kupcu S (2015) Anisotropic crystalline protein Nanolayers as multi-functional biointerface for patterned co-cultures of adherent and non-adherent cells in microfluidic devices. Adv Mater Interfaces 2(1):ARTN 1400309.  https://doi.org/10.1002/admi.201400309CrossRefGoogle Scholar
  12. 12.
    Ertl P, Sticker D, Charwat V, Kasper C, Lepperdinger G (2014) Lab-on-a-chip technologies for stem cell analysis. Trends Biotechnol 32(5):245–253.  https://doi.org/10.1016/j.tibtech.2014.03.004CrossRefPubMedGoogle Scholar
  13. 13.
    Sticker D, Rothbauer M, Lechner S, Hehenberger MT, Ertl P (2015) Multi-layered, membrane-integrated microfluidics based on replica molding of a thiol-ene epoxy thermoset for organ-on-a-chip applications. Lab Chip 15(24):4542–4554.  https://doi.org/10.1039/c5lc01028dCrossRefPubMedGoogle Scholar
  14. 14.
    Charwat V, Purtscher M, Tedde SF, Hayden O, Ertl P (2013) Standardization of microfluidic cell cultures using integrated organic photodiodes and electrode arrays. Lab Chip 13(5):785–797.  https://doi.org/10.1039/c2lc40965hCrossRefPubMedGoogle Scholar
  15. 15.
    Rosser J, Ertl P (2016) 3D microfluidic cell culture models for potential stem cell therapies. Insights in Stem CellsGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Faculty of Technical Chemistry, Institute of Applied Synthetic ChemistryVienna University of TechnologyViennaAustria
  2. 2.Faculty of Technical Chemistry, Institute of Chemical Technologies and Analytics (CTA)Vienna University of TechnologyViennaAustria

Personalised recommendations