Abstract
Chlorophyll fluorescence is a rapid and non-invasive tool used for probing the activity of photosynthesis that can be used in vivo and in the field. It is highly relevant to the demands of high-throughput crop phenotyping and can be automated or manually applied. Here we describe protocols and advice for making fast timescale fluorescence measurements using handheld equipment in the laboratory or in the field. While interpretation of some measured parameters requires caution, we demonstrate that this technique is appropriate for some applications where convenience, rapidity, and sensitivity are required.
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References
Pask AJD, Pietragalla J, Mullan D, Reynolds MP (eds) (2012) Physiological breeding II: a field guide to wheat phenotyping. Mexico, International Wheat and Maize Improvement Center (CIMMYT)
Schreiber U, Schliwa U, Bilger W (1986) Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulated fluorometer. Photosynth Res 10:51–62
Strasser RJ, Govindjee (1991) The F0 and the O-J–I–P fluorescence rise in higher plants and algae. In: Argyroudi-Akoyunoglou JH (ed) Regulation of chloroplast biogenesis. Plenum Press, New York, pp 423–426
Murchie EH, Lawson T (2013) Chlorophyll fluorescence analysis: a guide to good practice and understanding some new applications. J Exp Bot 64:3983–3998
Lazár D (2006) The polyphasic chlorophyll a fluorescence rise measured under high intensity of exciting light. Funct Plant Biol 33:9–30
Strasser RJ, Tsimilli-Michael M, Srivastava A (2004) Analysis of the chlorophyll a fluorescence transient. In: Papageorgiou GC, Govindjee (eds) Chlorophyll a fluorescence: a signature of photosynthesis. Kluwer Academic Publishers, Dordrecht, pp 321–362
Strasser RJ, Srivastava A, Govindjee (1995) Polyphasic chlorophyll a fluorescence transients in plants and cyanobacteria. Photochem Photobiol 61:32–42
Ajigboye OO, Murchie EH, Ray RV (2014) Foliar application of isopyrazam and epoxiconazole improves photosystem II efficiency, biomass and yield in winter wheat. Pestic Biochem Physiol 114:52–60
Ajigboye OO, Bousquet L, Murchie EH, Ray RV (2016) Chlorophyll fluorescence parameters allow the rapid detection and differentiation of plant responses in three different wheat pathosystems. Funct Plant Biol 43(4):356–369
Ajigboye OO, Lu C, Murchie EH, Schlatter C, Swart G, Ray RV (2017) Altered gene expression by sedaxane increases PSII efficiency, photosynthesis and growth and improves tolerance to drought in wheat seedlings. Pestic Biochem Physiol 137:49–61
Stirbet A, Govindjee (2011) On the relation between the Kautsky effect (chlorophyll a fluorescence induction) and photosystem II: basics and applications of the OJIP fluorescence transient. J Photochem Photobiol B 104:236–257
Strasser RJ, Srivastava A, Tsimilli-Michael M (2000) The fluorescence transient as a tool to characterize and screen photosynthetic samples. In: Yunus M, Pathre U, Mohanty P (eds) Probing photosynthesis: mechanism, regulation and adaptation, vol 25. Taylor and Francis, London, pp 445–483
Suzuki S, Nakamoto H, Ku MSB, Edwards GE (1987) Influence of leaf age on photosynthesis, enzyme activity, and metabolite levels in wheat. Plant Physiol 84:1244–1248
Ripoll J, Bertin N, Bidel LPR, Urban L (2016) A user’s view of the parameters derived from the induction curves of maximal chlorophyll a fluorescence: perspectives for analysing stress. Front Plant Sci 7:1679
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Ajigboye, O.O., Ray, R.V., Murchie, E.H. (2018). Chlorophyll Fluorescence on the Fast Timescale. In: Covshoff, S. (eds) Photosynthesis. Methods in Molecular Biology, vol 1770. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7786-4_6
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DOI: https://doi.org/10.1007/978-1-4939-7786-4_6
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