Skip to main content
SpringerLink
Log in

Photosynthetic Gas Exchange in Land Plants at the Leaf Level

  • Protocol
  • First Online:
Photosynthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1770))

Abstract

Leaf-level gas exchange enables insights into the physiology and in vivo biochemical processes of plants. Advances in infrared gas analysis have resulted in user-friendly off-the-shelf gas exchange systems that allow researchers to collect physiological measurements with the push of a few buttons. Here, I describe how to set up the gas exchange equipment and what to pay attention to while making measurements, and provide some guidelines on how to analyze and interpret the data obtained.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 109.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 139.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info
Hardcover Book
USD 199.99
Price excludes VAT (USA)
  • Durable hardcover edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Li-Cor (2012) Using the LI-6400 / LI-6400XT portable photosynthesis system, version 6. Li-Cor Biosciences, Lincoln, NE

    Google Scholar 

  2. Walz (2013) Portable gas exchange fluorescence system GFS-3000, 7th edn. Heinz Walz GmbH, Effeltrich

    Google Scholar 

  3. Li-Cor (2016) Using the LI-6800 portable photosynthesis system, version 1. Li-Cor Biosciences, Lincoln, NE

    Google Scholar 

  4. Long SP, Bernacchi CJ (2003) Gas exchange measurements, what can they tell us about the underlying limitations to photosynthesis? Procedures and sources of error. J Exp Bot 54(392):2393–2401

    Article  CAS  PubMed  Google Scholar 

  5. Evans JR, Santiago LS (2014) PrometheusWiki Gold Leaf Protocol: gas exchange using LI-COR 6400. Funct Plant Biol 41(3):223–226. https://doi.org/10.1071/FP10900

    Article  CAS  Google Scholar 

  6. Sharkey TD (2016) What gas exchange data can tell us about photosynthesis. Plant Cell Environ 39(6):1161–1163. https://doi.org/10.1111/pce.12641

    Article  PubMed  CAS  Google Scholar 

  7. Farquhar GD, von Caemmerer S, Berry JA (1980) A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. Planta 149(1):78–90

    Article  CAS  PubMed  Google Scholar 

  8. von Caemmerer S (2000) Biochemical models of leaf photosynthesis. CSIRO Publishing, Collingwood

    Google Scholar 

  9. Harley PC, Sharkey TD (1991) An improved model of C3 photosynthesis at high CO2: Reversed O2 sensitivity explained by lack of glycerate reentry into the chloroplast. Photosynth Res 27(3):169–178

    PubMed  CAS  Google Scholar 

  10. Flexas J, Barbour MM, Brendel O, Cabrera HM, Carriquí M, Díaz-Espejo A, Douthe C, Dreyer E, Ferrio JP, Gago J, Gallé A, Galmés J, Kodama N, Medrano H, Niinemets Ü, Peguero-Pina JJ, Pou A, Ribas-Carbó M, Tomás M, Tosens T, Warren CR (2012) Mesophyll diffusion conductance to CO2: An unappreciated central player in photosynthesis. Plant Sci 193–194:70–84. https://doi.org/10.1016/j.plantsci.2012.05.009

    Article  PubMed  CAS  Google Scholar 

  11. Sharkey TD, Bernacchi CJ, Farquhar GD, Singsaas EL (2007) Fitting photosynthetic carbon dioxide response curves for C3 leaves. Plant Cell Environ 30(9):1035–1040. https://doi.org/10.1111/j.1365-3040.2007.01710.x

    Article  PubMed  CAS  Google Scholar 

  12. Mott KA, Peak D (2011) Alternative perspective on the control of transpiration by radiation. Proc Natl Acad Sci U S A 108(49):19820–19823. https://doi.org/10.1073/pnas.1113878108

    Article  PubMed  PubMed Central  Google Scholar 

  13. Duursma RA (2015) Plantecophys - An R package for analysing and modelling leaf gas exchange data. PLoS One 10(11):e0143346. https://doi.org/10.1371/journal.pone.0143346

    Article  PubMed  PubMed Central  CAS  Google Scholar 

  14. Dubois JJB, Fiscus EL, Booker FL, Flowers MD, Reid CD (2007) Optimizing the statistical estimation of the parameters of the Farquhar-von Caemmerer-Berry model of photosynthesis. New Phytol 176:402–414. https://doi.org/10.1111/j.1469-8137.2007.02182.x

    Article  PubMed  Google Scholar 

  15. Gu LH, Pallardy SG, Tu K, Law BE, Wullschleger SD (2010) Reliable estimation of biochemical parameters from C3 leaf photosynthesis-intercellular carbon dioxide response curves. Plant Cell Environ 33(11):1852–1874. https://doi.org/10.1111/j.1365-3040.2010.02192.x

    Article  PubMed  CAS  Google Scholar 

  16. Busch FA, Sage RF (2017) The sensitivity of photosynthesis to O2 and CO2 concentration identifies strong Rubisco control above the thermal optimum. New Phytol 213(3):1036–1051. https://doi.org/10.1111/nph.14258

    Article  PubMed  CAS  Google Scholar 

  17. Ethier GJ, Livingston NJ (2004) On the need to incorporate sensitivity to CO2 transfer conductance into the Farquhar-von Caemmerer-Berry leaf photosynthesis model. Plant Cell Environ 27(2):137–153

    Article  CAS  Google Scholar 

  18. Flexas J, Diaz-Espejo A, Berry JA, Cifre J, Galmes J, Kaidenhoff R, Medrano H, Ribas-Carbo M (2007) Analysis of leakage in IRGA's leaf chambers of open gas exchange systems: quantification and its effects in photosynthesis parameterization. J Exp Bot 58(6):1533–1543. https://doi.org/10.1093/jxb/erm027

    Article  PubMed  CAS  Google Scholar 

  19. Rodeghiero M, Niinemets U, Cescatti A (2007) Major diffusion leaks of clamp-on leaf cuvettes still unaccounted: how erroneous are the estimates of Farquhar et al. model parameters? Plant Cell Environ 30(8):1006–1022. https://doi.org/10.1111/j.1365-3040.2007.001689.x

    Article  PubMed  CAS  Google Scholar 

  20. Stinziano JR, Morgan PB, Lynch DJ, Saathoff AJ, McDermitt DK, Hanson DT (2017) The rapid A–Ci response: photosynthesis in the phenomic era. Plant Cell Environ 40(8):1256–1262. https://doi.org/10.1111/pce.12911

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

I thank Ross Deans for helpful comments on this chapter.

Author information

Authors and Affiliations

  1. Research School of Biology and ARC Centre of Excellence for Translational Photosynthesis, The Australian National University, Acton, ACT, Australia

    Florian A. Busch

Authors
  1. Florian A. Busch
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Florian A. Busch .

Editor information

Editors and Affiliations

  1. Niceville, Florida, USA

    Sarah Covshoff

Rights and permissions

Reprints and permissions

Copyright information

© 2018 Springer Science+Business Media, LLC, part of Springer Nature

About this protocol

Check for updates. Verify currency and authenticity via CrossMark

Cite this protocol

Busch, F.A. (2018). Photosynthetic Gas Exchange in Land Plants at the Leaf Level. In: Covshoff, S. (eds) Photosynthesis. Methods in Molecular Biology, vol 1770. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7786-4_2

Download citation

  • DOI: https://doi.org/10.1007/978-1-4939-7786-4_2

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7785-7

  • Online ISBN: 978-1-4939-7786-4

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics

Search

Navigation