Abstract
This chapter compares two different techniques for monitoring photosynthetic O2 production: the widespread Clark-type O2 electrode and the more sophisticated membrane inlet mass spectrometry (MIMS) technique. We describe how a simple membrane inlet for MIMS can be made out of a commercial Clark-type cell, and outline the advantages and drawbacks of the two techniques to guide researchers in deciding which method to use. Protocols and examples are given for measuring O2 evolution rates and for determining the number of chlorophyll molecules per active photosystem II reaction center.
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Acknowledgments
We thank Dr. Mun Hon Cheah for careful reading of the manuscript and valuable suggestions. The Swedish Energy Agency (Energimyndigheten), Swedish Science Foundation (Vetenskapsrådet), and the K & A Wallenberg Foundation are acknowledged for financial support.
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Shevela, D., Schröder, W.P., Messinger, J. (2018). Liquid-Phase Measurements of Photosynthetic Oxygen Evolution. In: Covshoff, S. (eds) Photosynthesis. Methods in Molecular Biology, vol 1770. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7786-4_11
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DOI: https://doi.org/10.1007/978-1-4939-7786-4_11
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