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Survey of Tools for Measuring In Vivo Photosynthesis

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Book cover Photosynthesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1770))

Abstract

Measurements of in vivo photosynthesis are powerful tools that probe the largest fluxes of carbon and energy in an illuminated leaf, but often the specific techniques used are so varied and specialized that it is difficult for researchers outside the field to select and perform the most useful assays for their research questions. The goal of this chapter is to provide a broad overview of the current tools available for the study of in vivo photosynthesis so as to provide a foundation for selecting appropriate techniques, many of which are presented in detail in subsequent chapters. This chapter also organizes current methods into a comparative framework and provides examples of how they have been applied to research questions of broad agronomical, ecological, or biological importance. The chapter closes with an argument that the future of in vivo measurements of photosynthesis lies in the ability to use multiple methods simultaneously and discusses the benefits of this approach to currently open physiological questions. This chapter, combined with the relevant methods chapters, could serve as a laboratory course in methods in photosynthesis research or as part of a more comprehensive laboratory course in general plant physiology methods.

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Authors and Affiliations

  1. Biochemistry of Plants, Heinrich-Heine University, Düsseldorf, Germany

    Berkley J. Walker

  2. Research School of Biology and ARC Centre of Excellence for Translational Photosynthesis, The Australian National University, Acton, ACT, Australia

    Florian A. Busch

  3. Centre for Crop Systems Analysis, Wageningen University and Research, Wageningen, The Netherlands

    Steven M. Driever

  4. Carl R. Woese Institute for Genomic Biology, University of Illinois, Urbana, IL, USA

    Johannes Kromdijk

  5. School of Biological Sciences, University of Essex, Colchester, Essex, UK

    Tracy Lawson

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  1. Berkley J. Walker
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  2. Florian A. Busch
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  3. Steven M. Driever
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  4. Johannes Kromdijk
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  5. Tracy Lawson
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Correspondence to Berkley J. Walker .

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Editors and Affiliations

  1. Niceville, Florida, USA

    Sarah Covshoff

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Walker, B.J., Busch, F.A., Driever, S.M., Kromdijk, J., Lawson, T. (2018). Survey of Tools for Measuring In Vivo Photosynthesis. In: Covshoff, S. (eds) Photosynthesis. Methods in Molecular Biology, vol 1770. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7786-4_1

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  • DOI: https://doi.org/10.1007/978-1-4939-7786-4_1

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