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Chromothripsis pp 209–230Cite as

Study of Telomere Dysfunction in TP53 Mutant LoVo Cell Lines as a Model for Genomic Instability

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1769))

Abstract

Telomere restriction fragment, 3D quantitative FISH on nuclei, and quantitative FISH on metaphases are complementary approaches that explore telomere dysfunction genomically, cellularly, and chromosomally, respectively. We used these approaches to study association between telomere dysfunction and degree of genomic instability related to TP53 mutations in LoVo isogenic cell lines. We found a strong correlation between degree of genomic instability, telomere dysfunction, and specific mutations of TP53. The use of complementary approaches to study telomere biology is essential to have a comprehensive understanding of telomere involvement in genomic instability.

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Author information

Authors and Affiliations

  1. 3D Signatures Holdings Inc. MaRS Centre, South Tower, Toronto, ON, Canada

    Oumar Samassekou

  2. Laboratoire d’Anatomopathologie et de Cytologie, Laboratoires médicaux de la Capitale Nationale et des Îles, site IUCPQ-UL, 2725 Chemin Sainte-Foy, Québec, QC, Canada

    Nathalie Bastien

  3. Cytogenetics and Molecular Cytogenetics laboratory, Beijing Boren Hospital, Beijing, China

    Ju Yan

  4. Department of Physiology and Pathophysiology, Cell Biology, University of Manitoba, Research Institute of Oncology and Hematology, CancerCare Manitoba, Winnipeg, MB, Canada

    Sabine Mai

  5. Division of Medical Genetics, Department of Pediatrics, Laval University and Centre Hospitalier Universitaire de Québec, Quebec City, QC, Canada

    Régen Drouin

Authors
  1. Oumar Samassekou
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  2. Nathalie Bastien
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  3. Ju Yan
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  4. Sabine Mai
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  5. Régen Drouin
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Corresponding author

Correspondence to Régen Drouin .

Editor information

Editors and Affiliations

  1. Unit of Chromosomal Genetics, Department of Medical Genetics, CHU Montpellier, Montpellier, Hérault, France

    Franck Pellestor

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Samassekou, O., Bastien, N., Yan, J., Mai, S., Drouin, R. (2018). Study of Telomere Dysfunction in TP53 Mutant LoVo Cell Lines as a Model for Genomic Instability. In: Pellestor, F. (eds) Chromothripsis. Methods in Molecular Biology, vol 1769. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7780-2_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7780-2_14

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7779-6

  • Online ISBN: 978-1-4939-7780-2

  • eBook Packages: Springer Protocols

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