Common Protocols in Orchid Micropropagation

  • So-Young Park
  • Yoon-Sun Huh
  • Kee-Yoeup Paek
Part of the Springer Protocols Handbooks book series (SPH)


Orchids are popular plants because of the wide range of colors, shapes, sizes, and fragrances of their flowers. The demand for orchids as cut flowers and pot plants is ever increasing in the market. The survival and subsequent germination of orchid seeds are comparatively low in the wild. Thus, to meet the demand of the horticultural industry, the in vitro germination of orchid seeds needs to be performed. Although tropical orchids can germinate on relatively simple media, the temperate orchids might require complex media or the presence of a symbiotic fungus. This chapter focuses on two common micropropagation methods using seed and protocorm-like body (PLB) cultures, respectively. For seed culture, the micropropagation of Cypripedium macranthos is detailed. Regeneration of orchids can also be achieved using protocorms or PLBs from vegetative explants. PLBs are excellent explants for clonal propagation, artificial seed development, and genetic engineering because they are organized and easily regenerable somatic embryos that propagate rapidly. Here, the development of PLBs from leaf sections and root tips of Phalaenopsis orchids is also described.

Key words

Orchids Seed culture Protocorm Protocorm-like bodies Leaf thin sections Root tip culture Cypripedium macranthos Phalaenopsis 


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© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Horticultural ScienceChungbuk National UniversityCheongju-siRepublic of Korea
  2. 2.Chungbuk Agricultural Research and Extension ServicesCheongju-siRepublic of Korea

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