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The Use of Laser Microdissection to Investigate Cell-Specific Gene Expression in Orchid Tissues

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Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols

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Abstract

In the past decade, laser microdissection (LMD) technology has been widely applied to plant tissues, highlighting the role of different cell-type populations during plant–microbe interactions. In this chapter, a method to apply the LMD approach to study gene expression in specific cell-type populations of orchid mycorrhizal protocorms and roots is described in detail, starting from the preparation of biological material to gene expression analysis by RT-PCR.

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Acknowledgments

The LMD system upgrade was possible thanks to the financial support of the “Compagnia di San Paolo” (Torino, Italy).

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Authors and Affiliations

  1. Istituto per la Protezione Sostenibile delle Piante, Consiglio Nazionale delle Ricerche (CNR), Turin, Italy

    Raffaella Balestrini & Silvia Perotto

  2. Dipartimento di Scienze della Vita e Biologia dei Sistemi, Università degli Studi di Torino, Turin, Italy

    Valeria Fochi, Alessandro Lopa & Silvia Perotto

Authors
  1. Raffaella Balestrini
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  2. Valeria Fochi
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  3. Alessandro Lopa
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  4. Silvia Perotto
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Corresponding author

Correspondence to Raffaella Balestrini .

Editor information

Editors and Affiliations

  1. Biology Department, National Museum of Natural Science, Taichung, China

    Yung-I Lee

  2. Department of Biological Sciences, University of Calgary, Calgary, Alberta, Canada

    Edward Chee-Tak Yeung

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Balestrini, R., Fochi, V., Lopa, A., Perotto, S. (2018). The Use of Laser Microdissection to Investigate Cell-Specific Gene Expression in Orchid Tissues. In: Lee, YI., Yeung, ET. (eds) Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7771-0_21

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  • DOI: https://doi.org/10.1007/978-1-4939-7771-0_21

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7770-3

  • Online ISBN: 978-1-4939-7771-0

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