Abstract
Orchids are appreciated for their delicate and enchantingly beautiful floral parts. Decades ago, orchids were more expensive than other ornamentals due to their limited supplies and difficulties in propagation and cultivation. Nowadays, using modern tissue culture technology, orchids are readily available commercially, and the price of plants is becoming more affordable. However, during mass propagation, virulent viruses may also be reproduced along with tissue-cultured clones and thus decrease the growth vigor and the ornamental value of the finished products. As a result, virus detection prior to orchid propagation is a vital process in modern orchid industry, in order to prevent viruses from spreading among the tissue-cultured plantlets. This chapter describes a technique, namely, an enzyme-linked immunosorbent assay (ELISA), which is currently widely adopted by most major orchid companies as a routine test for virus detection. The basic mechanism of the technique, materials and facilities needed, detailed protocols, result interpretation, and some tips to prevent background problems are presented in the text. We also detail and discuss some alternative methods that orchid growers can use in orchid virus detection.
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Chang, CA. (2018). Virus Detection. In: Lee, YI., Yeung, ET. (eds) Orchid Propagation: From Laboratories to Greenhouses—Methods and Protocols. Springer Protocols Handbooks. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7771-0_18
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DOI: https://doi.org/10.1007/978-1-4939-7771-0_18
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