Haploid Seed Formation via Parthenogenesis in Bletilla

  • Juntaro Kato
  • Syoichi Ichihashi
Part of the Springer Protocols Handbooks book series (SPH)


This chapter provides a protocol to induce haploid plant formation in Bletilla via parthenogenesis by the application of 1-naphthaleneacetic acid sodium salt through the stigmatic surface of the column. Although the seed number is low, in vitro seed germination results in the formation of viable seedlings, and the ploidy levels as determined using flow-cytometric analyses indicate the formation of haploid and diploid (doubled haploid) plants. The successful induction of haploid plants through parthenogenesis can be a powerful tool in orchid breeding.

Key words

Bletilla Haploids Stigma 1-Naphthaleneacetic acid sodium salt Parthenogenesis Flow cytometry 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of BiologyAichi University of EducationKariyaJapan

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