In Vitro Flowering and Breeding of Erycina pusilla

Protocol
Part of the Springer Protocols Handbooks book series (SPH)

Abstract

Erycina pusilla is a miniature orchid species native to tropical America. The unique characteristics, e.g., a short juvenile stage and its miniature size, make E. pusilla a perfect material for producing the ornamental flask product and for the study of flowering physiology and in vitro breeding. In this chapter, we demonstrate the culture steps of in vitro flowering of E. pusilla. Using method detailed, it is possible to produce blooming E. pusilla plants within 1 year from in vitro seed germination and producing ornamental flasks for commercial sale. This culture protocol can also be used for in vitro breeding to select additional desirable traits for ornamental flask production.

Key words

Erycina pusilla flowering Miniature orchids Breeding Pollination Subcultured In vitro ornamental orchid product 

Reference

  1. 1.
    Murthy KSR, Kondamudi R, Chalapathi RPV, Pullaiah T (2012) In vitro flowering-a review. J Agric Technol 8:1517–1536Google Scholar
  2. 2.
    Chang C, Chang WC (2003) Cytokinins promotion of flowering in Cymbidium ensifolium var. misericors in vitro. Plant Growth Regul 39:217–221CrossRefGoogle Scholar
  3. 3.
    Vaz APA, Càssia RD, Figueiredo-Ribeiro L, Kerbauy GB (2004) Photoperiod and temperature effects on in vitro growth and flowering of P. pusilla, an epiphytic orchid. Plant Physiol Biochem 42:411–415CrossRefGoogle Scholar
  4. 4.
    Teixeira da Silva JA, Kerbauy GB, Zeng S, Chen Z, Duan J (2014) In vitro flowering of orchids. Crit Rev Biotechnol 34:56–76CrossRefGoogle Scholar
  5. 5.
    Lopez RG, Runkle ES (2005) Environmental physiology of growth and flowering of orchids. HortSci 40:1969–1973Google Scholar
  6. 6.
    Hew CS, Yong JWH (1997) Physiology of tropical orchids in relation to the industry. World Scientific Publishing Co. Pte Ltd, SingaporeCrossRefGoogle Scholar
  7. 7.
    Williams NH, Chase MW, Fulcher T, Whitten WM (2001) Molecular systematics of the Oncidiinae based on evidence from four DNA sequence regions: expanded circumscriptions of Cyrtochilum, Erycina, Otoglossum, and Trichocentrum and a new genus (Orchidaceae). Lindleyana 16:113–139Google Scholar
  8. 8.
    Dodson CH, Dressler RL (1972) Two undescribed genera in the Orchidaceae - Oncidiinae. Phytologia 24:285–292Google Scholar
  9. 9.
    Dodson CH (1957) Oncidium pusillum and its allies. Amer Orchid Soc Bull 26:170–172Google Scholar
  10. 10.
    Livingston R b (1962) Oncidium pusillum- blooming in flask! Amer Orchid Soc Bulletin 31:1007Google Scholar
  11. 11.
    Vaz APA, Kerbauy GP (2000) Effects of mineral nutrients on in vitro growth and flower formation of Psygmorchis pusilla (Orchidaceae). Acta Hort 520:149–156CrossRefGoogle Scholar
  12. 12.
    Vaz APA, Kerbauy GB (2008) In vitro flowering studies in Psygmorchis pusilla. Glo Sci 42:427–432Google Scholar
  13. 13.
    Baker ML, Baker CO (2006) Orchid species culture: oncidium/odontoglossum alliance. Timber Press, Portland, OR, p 992Google Scholar
  14. 14.
    Chiu YT, Lin CS, Chang C (2011) In vitro fruiting and seed production in Erycina papilla (L.) N.H. Williams & M.W. Chase. Prop Ornamental Plants 11:131–136Google Scholar
  15. 15.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of HorticultureNational Chung Hsing UniversityTaichung CityRepublic of China

Personalised recommendations