Infinium DNA Methylation Microarrays on Formalin-Fixed, Paraffin-Embedded Samples
Formalin-fixed, paraffin-embedded (FFPE) samples usually yield fragmented DNA, which is incompatible with traditional Infinium Methylation beadchips. In this chapter, we aim to explain in detail all the processes carried out in order to obtain high-quality methylation profiles from FFPE samples through an FFPE restoration procedure. High-throughput methylation profiling platforms such as the Infinium Methylation beadchips have been extensively used by the scientific community to elucidate many hypotheses in multiple scenarios, now with the incorporation of FFPE samples to these platforms, a myriad of new opportunities is being opened.
Key wordsEpigenetics DNA methylation Formalin-fixed, paraffin-embedded FFPE Methylation Microarray Restoration Degraded DNA
- 6.Moran S, Vizoso M, Martinez-Cardús A, Gomez A, Matías-Guiu X, Chiavenna SM, Fernandez AG, Esteller M (2014) Validation of DNA methylation profiling in formalin-fixed paraffin-embedded samples using the Infinium HumanMethylation450 Microarray. Epigenetics 9:829–833CrossRefPubMedPubMedCentralGoogle Scholar