Analysis of Chromatin Interactions Mediated by Specific Architectural Proteins in Drosophila Cells
Chromosome conformation capture assays have been established, modified, and enhanced for over a decade with the purpose of studying nuclear organization. A recently published method uses in situ Hi-C followed by chromatin immunoprecipitation (HiChIP) to enrich the overall yield of significant genome-wide interactions mediated by a specific protein. Here we applied a modified version of the HiChIP protocol to retrieve the significant contacts mediated by architectural protein CP190 in D. melanogaster cells.
Key wordsChromatin immunoprecipitation Chromatin architecture In situ Hi-C HiChIP Epigenetics Transcription
This work was supported by US Public Health Service Award R01 GM035463 from the National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health. Masami Ando-Kuri and I. Sarahi M. Rivera contributed equally to this work.