Genome-Wide Mapping of Protein–DNA Interactions on Nascent Chromatin
Chromatin immunoprecipitation (ChIP) is the most widely used method to analyze protein–DNA interactions in vivo. Coupled with next generation sequencing, ChIP-seq experiments map protein–DNA interactions in a genome-wide fashion. Here we describe a novel method called nasChIP-seq for mapping genome-wide occupancy of posttranslationally modified histones or transcription factors on newly replicated DNA.
Key wordsChromatin immunoprecipitation ChIP-seq DNA replication Nascent chromatin Click chemistry
Work in the authors’ laboratory was supported by U.S. Public Health Service Award R01 GM035463 from the National Institutes of Health. The content is solely the responsibility of the authors and does not necessarily represent the official views of the National Institutes of Health.
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