Identification of Response Elements on Promoters Using Site-Directed Mutagenesis and Chromatin Immunoprecipitation
Proximal promoters are located upstream of the transcription start sites of genes, and they contain regulatory sequences on which bind different transcription factors for promoting colorectal cancer progression. Here we describe the comprehensive methodology used previously for the identification and functional characterization of MYC-responsive elements in the integrin α1 subunit (ITGA1) gene using a combination of in silico analysis, site-directed mutagenesis, and chromatin immunoprecipitation.
Key wordsPromoters ChIP Response element Mutagenesis Transcription factor MYC ITGA1
The original work was supported by the Canadian Institute of Health Research Grants MOP-97836 and MOP-123415. J-FB was the recipient of the Canadian Research Chair in Intestinal Physiopathology. JCC is a scholar of the Fonds de la Recherche du Québec-Santé (FRQS). SB is a recipient of the FRQS postdoctoral fellowship.
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