Identification of Response Elements on Promoters Using Site-Directed Mutagenesis and Chromatin Immunoprecipitation

  • Salah Boudjadi
  • Julie C. Carrier
  • Jean-François Beaulieu
Part of the Methods in Molecular Biology book series (MIMB, volume 1765)


Proximal promoters are located upstream of the transcription start sites of genes, and they contain regulatory sequences on which bind different transcription factors for promoting colorectal cancer progression. Here we describe the comprehensive methodology used previously for the identification and functional characterization of MYC-responsive elements in the integrin α1 subunit (ITGA1) gene using a combination of in silico analysis, site-directed mutagenesis, and chromatin immunoprecipitation.

Key words

Promoters ChIP Response element Mutagenesis Transcription factor MYC ITGA1 



The original work was supported by the Canadian Institute of Health Research Grants MOP-97836 and MOP-123415. J-FB was the recipient of the Canadian Research Chair in Intestinal Physiopathology. JCC is a scholar of the Fonds de la Recherche du Québec-Santé (FRQS). SB is a recipient of the FRQS postdoctoral fellowship.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Salah Boudjadi
    • 1
  • Julie C. Carrier
    • 2
  • Jean-François Beaulieu
    • 3
  1. 1.Cancer Molecular Pathology Section, Laboratory of PathologyNational Cancer InstituteBethesdaUSA
  2. 2.Department of Medicine, Faculty of Medicine and Health ScienceUniversité de SherbrookeSherbrookeCanada
  3. 3.Laboratory of Intestinal Physiopathology, Department of Anatomy and Cell Biology, Faculty of Medicine and Health SciencesUniversité de SherbrookeSherbrookeCanada

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