Use of Organoids to Characterize Signaling Pathways in Cancer Initiation

  • Christina Oatway
  • Calley L. Hirsch
  • Alex Gregorieff
Part of the Methods in Molecular Biology book series (MIMB, volume 1765)


The development of intestinal organoid technology has greatly accelerated research in the field of colorectal cancer. Contrary to traditional cancer cell lines, organoids are composed of multiple cell types arranged in 3D structures highly reminiscent of their native tissues. Thus, organoids provide a near-physiological and readily accessible model to study tissue morphogenesis, adult stem cell behavior and tumorigenesis. Here, we provide protocols for establishing intestinal organoid cultures from genetically modified mouse lines and describe methods to overexpress and knockout genes of interest using lentiviral-based approaches.

Key words

Organoid Intestinal stem cells Wnt Apc Yap Lentiviral infection 


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Christina Oatway
    • 1
  • Calley L. Hirsch
    • 1
  • Alex Gregorieff
    • 1
    • 2
    • 3
  1. 1.Lunenfeld-Tanenbaum Research Institute, Sinai Health SystemTorontoCanada
  2. 2.Department of PathologyMcGill UniversityMontrealCanada
  3. 3.Cancer Research Program of the Research Institute of McGill University Health CentreMontrealCanada

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