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Tumor-Derived Microparticles to Monitor Colorectal Cancer Evolution

  • Diane Mege
  • Laurence Panicot-Dubois
  • Christophe Dubois
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1765)

Abstract

Colorectal cancer is the most common gastrointestinal cancer and the second-most common cause of cancer-related death worldwide. Microparticles are small vesicles known to be implicated in tumor growth, the development of metastases, and cancer-associated procoagulant activity. We have reported a specific hallmark of microparticles in colorectal and pancreatic cancers, the so-called “microparticulosome.” We have also reported on variations in the microparticulosome in the case of the occurrence of a thromboembolic event. Here, we describe our flow cytometry-based methods for identifying and characterizing tumor-derived microparticles and thereby monitoring colorectal cancer evolution.

Key words

Microparticle Tumor-derived microvesicle Colorectal cancer Tissue factor Fibrin 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Diane Mege
    • 1
  • Laurence Panicot-Dubois
    • 1
  • Christophe Dubois
    • 1
  1. 1.Aix Marseille University, INSERM UMR-S1076, C2VNMarseilleFrance

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