Autophagic Flux Assessment in Colorectal Cancer Cells

  • Annie Lauzier
  • Steve Jean
Part of the Methods in Molecular Biology book series (MIMB, volume 1765)


Autophagy protects colorectal cancer cells against therapeutic intervention. Autophagy is a continuous process, and autophagic flux requires both autophagosome synthesis and their subsequent degradation at lysosomes. Hence, cells with elevated autophagic flux display both rapid autophagosome generation and degradation. Here, we describe an immunoblot protocol coupled to pharmaceutical inhibition of autophagosome clearance to monitor autophagic flux levels between colorectal cancer cell lines.

Key words

Autophagy Colorectal cancer cells Immunoblots LC3 Bafilomycin A1 



This work was supported by grants from the Canadian Institutes of Health Research (CIHR) and the Cancer Research Society (CRS) to S.J. and by junior faculty salary awards from the CIHR and Fond de Recherche du Québec—Santé (FRQS) to S.J.. Steve Jean is a member of the FRSQ-Funded Centre de Recherche du CHUS.


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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  1. 1.Department of Anatomy and Cell Biology, Faculty of Medicine and Health SciencesUniversité de SherbrookeSherbrookeCanada

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