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Correlative 3D Structured Illumination Microscopy and Single-Molecule Localization Microscopy for Imaging Cancer Invasion

  • Shannon J L. Pinnington
  • John F. Marshall
  • Ann P. Wheeler
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1764)

Abstract

Super-resolution microscopy methods enable resolution of biological molecules in their cellular or tissue context at the nanoscale. Different methods have their strengths and weaknesses. Here we present a method that enables correlative confocal, structured illumination microscopy (SIM) and single-molecule localization microscopy (SMLM) imaging of structures involved in formation of invadopodia on the same sample. This enables up to four colors to be visualized in three dimensions at a resolution of between 120 and 10 nm for SIM and SMLM, respectively.

Key words

Invasion Microscopy Super-resolution Cells 

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Copyright information

© Springer Science+Business Media, LLC, part of Springer Nature 2018

Authors and Affiliations

  • Shannon J L. Pinnington
    • 1
  • John F. Marshall
    • 2
  • Ann P. Wheeler
    • 1
  1. 1.Advanced Imaging Resource, Institute of Genetics and Molecular MedicineUniversity of EdinburghEdinburghUK
  2. 2.Barts Cancer Institute, Barts and The London School of Medicine and DentistryQueen Mary University of LondonLondonUK

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