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Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies

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Protein Complex Assembly

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1764))

Abstract

Breaking the resolution limit of conventional microscopy by super-resolution microscopy (SRM) led to many new biological insights into protein assemblies at the nanoscale. Here we provide detailed protocols for single-molecule localization microscopy (SMLM) to image the structure of a protein complex. As examples, we show how to acquire single- and dual-color super-resolution images of the nuclear pore complex (NPC) and dual-color 3D data on actin and paxillin in focal adhesions.

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Acknowledgments

We thank Ulf Matti for experimental assistance and Edward Lemke for the Flp-In™ T-Rex™ 293 cell line. This work was supported by EMBL International PhD Programme fellowships (P.H. and M.M.).

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Authors and Affiliations

  1. Cell Biology and Biophysics Unit, European Molecular Biology Laboratory, Heidelberg, Germany

    Philipp Hoess, Markus Mund, Manuel Reitberger & Jonas Ries

Authors
  1. Philipp Hoess
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  2. Markus Mund
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  3. Manuel Reitberger
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  4. Jonas Ries
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Corresponding author

Correspondence to Jonas Ries .

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Editors and Affiliations

  1. MRC Human Genetics Unit, Institute of Genetics & Molecular Medicine, University of Edinburgh, Edinburgh, United Kingdom

    Joseph A. Marsh

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Hoess, P., Mund, M., Reitberger, M., Ries, J. (2018). Dual-Color and 3D Super-Resolution Microscopy of Multi-protein Assemblies. In: Marsh, J. (eds) Protein Complex Assembly. Methods in Molecular Biology, vol 1764. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7759-8_14

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  • DOI: https://doi.org/10.1007/978-1-4939-7759-8_14

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7758-1

  • Online ISBN: 978-1-4939-7759-8

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