Abstract
Plant health and development are directly depending on a plant’s ability to react to a constantly changing environment. Sensing of water and nutrition levels and of the biotic environment is vital for a plant, making the root one of the key plant organs. Proteins are the key molecules that play numerous roles in a cell’s everyday life. Quantitative proteome profiling of roots can provide a global overview on the molecular regulatory mechanisms and networks involved in plant growth and development and abiotic and biotic stress responses. Here, we provide a detailed proteomics workflow on Arabidopsis thaliana roots from plant growth up to proteomics data analysis.
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Acknowledgments
L.D.V. is the recipient of a VIB International Ph.D. program fellowship. E.S. was a postdoctoral fellow of the Research Foundation-Flanders. Kris Gevaert and Ive De Smet contributed equally to this work.
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Nikonorova, N., Vu, L.D., Stes, E., Gevaert, K., De Smet, I. (2018). Proteome Analysis of Arabidopsis Roots. In: Ristova, D., Barbez, E. (eds) Root Development. Methods in Molecular Biology, vol 1761. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7747-5_20
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DOI: https://doi.org/10.1007/978-1-4939-7747-5_20
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