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Expression and Targeting of Tumor Markers in Gelfoam® Histoculture: Potential Individualized Assays for Immuno-Oncology

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3D Sponge-Matrix Histoculture

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1760))

Abstract

Tumor-specific antigens are important in the study of tumor biology, tumor diagnosis, and prognosis and as targets for tumor therapy. This chapter reviews patient colon, breast, and ovarian tumors in 3-dimensional Gelfoam® histoculture maintaining in vivo-like expression of the important tumor antigens, for example TAG-72 and CEA. We have also reviewed that fluorescent antibodies can target tumors in Gelfoam® histoculture, thereby providing an assay for individual patients for sensitivity to therapeutic antibodies which have become so important in immuno-oncology and other cancer therapies.

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Author information

Authors and Affiliations

  1. AntiCancer, Inc., San Diego, CA, USA

    Robert M. Hoffman

  2. Department of Surgery, UCSD, San Diego, CA, USA

    Robert M. Hoffman

  3. San Raffaele Roma Open University, Rome, Italy

    Fiorella Guadagni

  4. IRCCS San Raffaele Pisana, Rome, Italy

    Fiorella Guadagni

Authors
  1. Robert M. Hoffman
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  2. Fiorella Guadagni
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Corresponding author

Correspondence to Robert M. Hoffman .

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Editors and Affiliations

  1. AntiCancer, Inc., San Diego, California, USA

    Robert M. Hoffman

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Hoffman, R.M., Guadagni, F. (2018). Expression and Targeting of Tumor Markers in Gelfoam® Histoculture: Potential Individualized Assays for Immuno-Oncology. In: Hoffman, R. (eds) 3D Sponge-Matrix Histoculture. Methods in Molecular Biology, vol 1760. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7745-1_4

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  • DOI: https://doi.org/10.1007/978-1-4939-7745-1_4

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7743-7

  • Online ISBN: 978-1-4939-7745-1

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