Abstract
Distant organ colonization by cancer cells is the governing step of metastasis. We review in this chapter the modeling and imaging of organ colonization by cancer cells in Gelfoam® histoculture. ANIP 973 lung cancer cells expressing green fluorescent protein (GFP) were injected intravenously into nude mice, whereby they formed brilliantly fluorescing metastatic colonies on the mouse lung. The seeded lung tissue was then excised and incubated in the three-dimensional Gelfoam® histoculture that maintained the critical features of progressive in vivo organ colonization. Tumor progression was continuously visualized by GFP fluorescence of individual cultures over a 52-day period, during which tumor colonies spread throughout the lung. Organ colonization was selective in Gelfoam® histoculture for lung cancer cells to grow on lung tissue, since no growth occurred on histocultured mouse liver tissue. The ability to support selective organ colonization in Gelfoam® histoculture and visualize tumor progression by GFP fluorescence allows the in vitro study of the governing processes of metastasis.
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Paget S (1889) The distribution of secondary growths in cancer of the breast. Lancet 133(3421):571–573
Kuo T, Kubota T, Watanabe M, Furukawa T, Teramoto T, Ishibiki K, Kitajima M, Moossa AR, Penman S, Hoffman RM (1995) Liver colonization competence governs colon cancermetastasis. Proc Natl Acad Sci U S A 92:12085–12089
Chishima T, Yang M, Miyagi Y, Li L, Tan Y, Baranov E, Shimada H, Moossa AR, Penman S, Hoffman RM (1997) Governing step of metastasis visualized in vitro. Proc Natl Acad Sci U S A 94:11573–11576
Zolotukhin S, Potter M, Hauswirth WW, Guy J, Muzycka N (1996) A “humanized” green fluorescent protein cDNA adapted for high-level expression in mammalian cells. J Virol 70:4646–4654
Chishima T, Miyagi Y, Wang X, Yang M, Tan Y, Shimada H, Moossa AR, Hoffman RM (1997) Metastatic patterns of lung cancer visualized live and in process by green fluorescence protein expression. Clin Exp Metastasis 15:547–552
Hoffman RM (2005) The multiple uses of fluorescent proteins to visualize cancer in vivo. Nature Reviews Cancer 5:796–806
Hoffman RM, Yang M (2006) Subcellular imaging in the live mouse. Nature Protocols 1:775–782
Hoffman RM, Yang M (2006) Color-coded fluorescence imaging of tumor-host interactions. Nature Protocols 1:928–935
Hoffman RM, Yang M (2006) Whole-body imaging with fluorescent proteins. Nature Protocols 1:1429–1438
Hoffman RM (2015) Application of GFP imaging in cancer. Laboratory Investigation 95:432–452
Hoffman RM, editor (2012) In vivo cellular imaging using fluorescent proteins. Methods and Protocols. Methods in Molecular Biology, Vol. 872, Springer Protocols (Humana Press). ISBN 978-1-61779-796-5
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Hoffman, R.M., Chishima, T. (2018). Imaging the Governing Step of Metastasis in Gelfoam® Histoculture. In: Hoffman, R. (eds) 3D Sponge-Matrix Histoculture. Methods in Molecular Biology, vol 1760. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7745-1_20
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DOI: https://doi.org/10.1007/978-1-4939-7745-1_20
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