Abstract
Gelfoam® histoculture provides a valuable tool for experimental studies of normal and pathological tissue physiology. It allows us to understand cell-cell interactions by mirroring their original spatial relationship within body tissues. Gelfoam® histoculture can be employed to model host-pathogen interactions mimicking in vivo conditions in vitro. In the present chapter, we describe a protocol to process and infect lymphoid tissue explants with HIV and maintain them in Gelfoam® histoculture at the liquid-air interface. The Gelfoam® histocultures with human immunodeficiency virus (HIV) type 1-infected tissues have been used to further understand the biology of early HIV-1 pathogenesis, as well as a novel ex vivo platform to test the efficacy and toxicity of antiviral drugs.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Biancotto A, Iglehart SJ, Vanpouille C, Condack CE, Lisco A, Ruecker E, Hirsch I, Margolis LB, Grivel JC (2008) HIV-1 induced activation of CD4+ T cells creates new targets for HIV-1 infection in human lymphoid tissue ex vivo. Blood 111:699–704
Lederman MM, Margolis L (2008) The lymph node in HIV pathogenesis. Semin Immunol 20:187–195
Harrison R (1910) The outgrowth of the nerve fiber as a mode of protoplasmic movement. J Exp Zool 9:787–846
Carrel A (1912) On the permanent life of tissues outside of the organism. J Exp Med 15:516–528
Hoffman RM (1991) Three-dimensional histoculture: origins and applications in cancer research. Cancer Cells 3:86–92
Glushakova S, Baibakov B, Margolis LB, Zimmerberg J (1995) Infection of human tonsil histocultures: a model for HIV pathogenesis. Nat Med 1:1320–1322
Glushakova S, Grivel JC, Fitzgerald W, Sylwester A, Zimmerberg J, Margolis LB (1998) Evidence for the HIV-1 phenotype switch as a causal factor in acquired immunodeficiency. Nat Med 4:346–349
Grivel JC, Margolis L (2009) Use of human tissue explants to study human infectious agents. Nat Protoc 4:256–269
Grivel JC, Margolis LB (1999) CCR5- and CXCR4-tropic HIV-1 are equally cytopathic for their T-cell targets in human lymphoid tissue. Nat Med 5:344–346
Grivel JC, Penn ML, Eckstein DA, Schramm B, Speck RF, Abbey NW, Herndier B, Margolis L, Goldsmith MA (2000) Human immunodeficiency virus type 1 coreceptor preferences determine target T-cell depletion and cellular tropism in human lymphoid tissue. J Virol 74:5347–5351
Lisco A, Vanpouille C, Tchesnokov EP, Grivel JC, Biancotto A, Brichacek B, Elliott J, Fromentin E, Shattock R, Anton P, Gorelick R, Balzarini J, McGuigan C, Derudas M, Gotte M, Schinazi RF, Margolis L (2008) Acyclovir is activated into a HIV-1 reverse transcriptase inhibitor in herpesvirus-infected human tissues. Cell Host Microbe 4:260–270
Vanpouille C, Lisco A, Introini A, Grivel JC, Munawwar A, Merbah M, Schinazi RF, Derudas M, McGuigan C, Balzarini J, Margolis L (2012) Exploiting the anti-HIV-1 activity of acyclovir: suppression of primary and drug-resistant HIV isolates and potentiation of the activity by ribavirin. Antimicrob Agents Chemother 56:2604–2611
Vanpouille C, Khandazhinskaya A, Karpenko I, Zicari S, Barreto-de-Souza V, Frolova S, Margolis L, Kochetkov S (2014) A new antiviral: chimeric 3TC-AZT phosphonate efficiently inhibits HIV-1 in human tissues ex vivo. Antiviral Res 109:125–131
Saba E, Grivel JC, Vanpouille C, Brichacek B, Fitzgerald W, Margolis L, Lisco A (2010) HIV-1 sexual transmission: early events of HIV-1 infection of human cervico-vaginal tissue in an optimized ex vivo model. Mucosal Immunol 3:280–290
Biancotto A, Brichacek B, Chen SS, Fitzgerald W, Lisco A, Vanpouille C, Margolis L, Grivel JC (2009) A highly sensitive and dynamic immunofluorescent cytometric bead assay for the detection of HIV-1 p24. J Virol Methods 157:98–101
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Introini, A., Fitzgerald, W., Vanpouille, C., Margolis, L. (2018). Histoculture and Infection with HIV of Functional Human Lymphoid Tissue on Gelfoam® . In: Hoffman, R. (eds) 3D Sponge-Matrix Histoculture. Methods in Molecular Biology, vol 1760. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7745-1_17
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7745-1_17
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7743-7
Online ISBN: 978-1-4939-7745-1
eBook Packages: Springer Protocols