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3D Sponge-Matrix Histoculture pp 125–131Cite as

Methionine Dependency Determination of Human Patient Tumors in Gelfoam® Histoculture

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1760))

Abstract

The elevated requirement of methionine by cancer cells (methionine dependence) is a general metabolic abnormality in cancer. Methionine-dependent cancer cells are unable to proliferate and arrest in the late S/G2 phase of the cell cycle when methionine is restricted in vitro or in vivo. Cell-cycle arrest in late S/G2 was used as a biomarker of methionine dependence for patient tumors in Gelfoam® histoculture. Human cancer patient tumors, including tumors of the colon, breast, ovary, prostate, and a melanoma, were observed to be methionine dependent in Gelfoam® histoculture based on cell cycle analysis. This simple method can be used to screen patient tumors for methionine dependence and then subsequently apply appropriate chemotherapy for these patients to target this cancer-specific metabolic abnormality.

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Authors and Affiliations

  1. AntiCancer Inc., San Diego, CA, USA

    Robert M. Hoffman

  2. Department of Surgery, University of California, San Diego, CA, USA

    Robert M. Hoffman

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  1. Robert M. Hoffman
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Correspondence to Robert M. Hoffman .

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  1. AntiCancer, Inc., San Diego, California, USA

    Robert M. Hoffman

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Hoffman, R.M. (2018). Methionine Dependency Determination of Human Patient Tumors in Gelfoam® Histoculture. In: Hoffman, R. (eds) 3D Sponge-Matrix Histoculture. Methods in Molecular Biology, vol 1760. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7745-1_13

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  • DOI: https://doi.org/10.1007/978-1-4939-7745-1_13

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7743-7

  • Online ISBN: 978-1-4939-7745-1

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