Abstract
Peptide self-assembly is an important field in biomaterials in which short peptides are designed to aggregate into nanostructures that often form hydrogels. These peptides are typically made using solid-phase peptide synthesis (SPPS), a technique in which amino acids are added sequentially to a growing chain. This technique has been used to synthesize peptides with more than 100 amino acids. However, self-assembling peptides are designed to aggregate in solution, which often reduces coupling efficiency during synthesis and makes purification more difficult. Here, an outline of solid-phase peptide synthesis is provided, along with steps that can be used to improve the synthetic yield and purification of self-assembling peptides for regenerative medicine applications.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Webber MJ, Appel EA, Meijer EW, Langer R (2016) Supramolecular biomaterials. Nat Mater 15:13–26
Hartgerink JD, Beniash E, Stupp SI (2001) Self-assembly and mineralization of peptide-amphiphile nanofibers. Science 294:1684–1688
Palomo JM (2014) Solid-phase peptide synthesis: an overview focused on the preparation of biologically relevant peptides. RSC Adv 4:32658–32672
Coin I, Beyermann M, Bienert M (2007) Solid-phase peptide synthesis: from standard procedures to the synthesis of difficult sequences. Nat Protoc 2:3247–3256
Cui H, Pashuck ET, Velichko YS et al (2010) Spontaneous and x-ray-triggered crystallization at long range in self-assembling filament networks. Science 327:555–559
Jayawarna V, Ali M, Jowitt TA et al (2006) Nanostructured hydrogels for three-dimensional cell culture through self-assembly of fluorenylmethoxycarbonyl–dipeptides. Adv Mater Weinheim 18:611–614
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2018 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Thomas Pashuck, E. (2018). Synthesis of Self-Assembling Peptide-Based Hydrogels for Regenerative Medicine Using Solid-Phase Peptide Synthesis. In: Chawla, K. (eds) Biomaterials for Tissue Engineering. Methods in Molecular Biology, vol 1758. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7741-3_14
Download citation
DOI: https://doi.org/10.1007/978-1-4939-7741-3_14
Published:
Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7739-0
Online ISBN: 978-1-4939-7741-3
eBook Packages: Springer Protocols