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Detection and Quantification of MicroRNAs in Esophageal Adenocarcinoma

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Book cover Esophageal Adenocarcinoma

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1756))

Abstract

MicroRNAs (miRNAs) are a class of small, noncoding RNAs that have been emerging as novel regulators in esophageal adenocarcinoma. Their role has been established in various aspects of esophageal adenocarcinoma including carcinogenesis, progression, treatment, and prognosis. Therefore, miRNA detection, profiling, and quantification have become extremely important for scientists and clinicians. As miRNAs are small, their detection can be challenging. There have been various methods developed to detect and/or quantify miRNAs. This chapter aims to introduce the fundamentals and methods of the most commonly used approaches including miRNA microarrays and quantitative real-time polymerase chain reaction (RT-qPCR) to detect and quantify miRNAs in esophageal adenocarcinoma.

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Author information

Authors and Affiliations

  1. Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia

    Moein Amin, Farhadul Islam, Vinod Gopalan & Alfred K. Lam

  2. Department of Biochemistry and Molecular Biology, University of Rajshahi, Rajshahi, Bangladesh

    Farhadul Islam

Authors
  1. Moein Amin
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  2. Farhadul Islam
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  3. Vinod Gopalan
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  4. Alfred K. Lam
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Corresponding author

Correspondence to Alfred K. Lam .

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Editors and Affiliations

  1. Cancer Molecular Pathology of School of Medicine, Griffith University, Gold Coast, Australia

    Alfred K. Lam

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Amin, M., Islam, F., Gopalan, V., Lam, A.K. (2018). Detection and Quantification of MicroRNAs in Esophageal Adenocarcinoma. In: Lam, A. (eds) Esophageal Adenocarcinoma. Methods in Molecular Biology, vol 1756. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7734-5_22

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  • DOI: https://doi.org/10.1007/978-1-4939-7734-5_22

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7733-8

  • Online ISBN: 978-1-4939-7734-5

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