Liquid Biopsy for Investigation of Cancer DNA in Esophageal Adenocarcinoma: Cell-Free Plasma DNA and Exosome-Associated DNA

  • Robert A. Smith
  • Alfred K. Lam
Part of the Methods in Molecular Biology book series (MIMB, volume 1756)


Liquid biopsy of cancers is an area of increasing interest in medical practice for the surveillance, management, and potential detection of malignant cells, using minimally invasive collection of body fluids. A liquid biopsy is particularly useful for metastatic cancers, which may be difficult to be sampled by core biopsy, due to difficulty of access or an occult location. Access to DNA shed from esophageal adenocarcinoma can enable the detection of mutations confirming the presence of malignant cells or the evolution of clonal lines with altered treatment response profiles. In this chapter, we detail a method for the isolation of cell-free DNA from blood plasma and DNA associated with exosomes in blood from patients with esophageal adenocarcinoma.

Key words

Liquid biopsy Cell-free DNA Exosome-associated DNA Cancer surveillance Cancer detection Cancer management Mutations 


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Copyright information

© Springer Science+Business Media, LLC 2018

Authors and Affiliations

  1. 1.Genomics Research Centre, Institute of Health and Biomedical Innovation, School of Biomedical ScienceQueensland University of TechnologyKelvin GroveAustralia
  2. 2.Cancer Molecular Pathology of School of MedicineGriffith UniversityGold CoastAustralia

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