Abstract
Microgliosis is a hallmark of degenerative processes in the retina. Reactive microglia migrate to the photoreceptor layer and the subretinal space during outer retinal degeneration. This process creates a toxic milieu where reactive microglia and dying photoreceptors recruit additional reactive phagocytes. This results in the release of a multitude of proinflammatory factors which accelerate photoreceptor demise. In this chapter, we outline in detail how to monitor microgliosis in the Fam161a-deficient mouse model of Retinitis Pigmentosa by performing immunohistochemical stainings of retinal cryosections and flat mounts using the marker Iba1. This protocol will serve as a guideline in evaluating microglia reactivity and localization in various mouse models of retinal degeneration.
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Dannhausen, K., Rashid, K., Langmann, T. (2018). Microglia Analysis in Retinal Degeneration Mouse Models. In: Tanimoto, N. (eds) Mouse Retinal Phenotyping. Methods in Molecular Biology, vol 1753. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7720-8_10
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DOI: https://doi.org/10.1007/978-1-4939-7720-8_10
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Publisher Name: Humana Press, New York, NY
Print ISBN: 978-1-4939-7719-2
Online ISBN: 978-1-4939-7720-8
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