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Live Imaging of Fetal Intra-abdominal Organs Using Two-Photon Laser-Scanning Microscopy

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Mouse Embryogenesis

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1752))

Abstract

The processes by which the intra-abdominal organ circulatory system develops in the embryo and during organogenesis are unclear. Previous studies have used fixed tissues to study the development of abdominal organ vasculature in the embryo; however, the intravital circulation of intra-abdominal organs in rodent fetal development has not been studied. This protocol describes a system that uses two-photon laser-scanning microscopy (TPLSM) for real-time observation and quantification of normal and pathologic live fetal intra-abdominal dynamics while the fetus is still connected to the mother via the umbilical cord.

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References

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Authors and Affiliations

  1. Translational Medicine Program, Division of General and Thoracic Surgery, The Hospital for Sick Children, Toronto, ON, Canada

    Yuhki Koike, Bo Li, Yong Chen, Hiromu Miyake, Carol Lee & Agostino Pierro

  2. Department of Gastrointestinal and Pediatric Surgery, Mie University Graduate School of Medicine, Tsu, Mie, Japan

    Yuhki Koike, Mikihiro Inoue, Keiichi Uchida & Masato Kusunoki

  3. Translational Medicine, The Hospital for Sick Children, Toronto, ON, Canada

    Lijun Chi

  4. Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada

    Lijun Chi

  5. Department of Gastroenterology, Hepatology and Nutrition, The Hospital for Sick Children, Toronto, ON, Canada

    Richard Wu

  6. The Hospital for Sick Children, University of Toronto, Toronto, ON, Canada

    Agostino Pierro

  7. Translational Medicine, The Hospital for Sick Children, 686 Bay Street, Toronto, Ontario, M5G0A4, Canada

    Paul Delgado-Olguin

  8. Department of Molecular Genetics, University of Toronto, 1 King’s College Cir, Toronto, ON, M5S 1A8, Canada

    Paul Delgado-Olguin

  9. Heart & Stroke Richard Lewar Centres of Excellence in Cardiovascular Research, 6 Queen’s Park Cres W, Toronto, ON, M5S3H2, Canada

    Paul Delgado-Olguin

Authors
  1. Yuhki Koike
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  2. Bo Li
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  3. Yong Chen
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  4. Hiromu Miyake
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  5. Carol Lee
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  6. Lijun Chi
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  7. Richard Wu
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  8. Mikihiro Inoue
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  9. Keiichi Uchida
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  10. Masato Kusunoki
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  11. Paul Delgado-Olguin
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  12. Agostino Pierro
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Corresponding author

Correspondence to Agostino Pierro .

Editor information

Editors and Affiliations

  1. Translational Medicine, The Hospital for Sick Children, Toronto, Ontario, Canada

    Paul Delgado-Olguin

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Cite this protocol

Koike, Y. et al. (2018). Live Imaging of Fetal Intra-abdominal Organs Using Two-Photon Laser-Scanning Microscopy. In: Delgado-Olguin, P. (eds) Mouse Embryogenesis. Methods in Molecular Biology, vol 1752. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7714-7_6

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  • DOI: https://doi.org/10.1007/978-1-4939-7714-7_6

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7713-0

  • Online ISBN: 978-1-4939-7714-7

  • eBook Packages: Springer Protocols

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