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Nitric Oxide pp 49–57Cite as

Quantum Cascade Lasers-Based Detection of Nitric Oxide

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Part of the book series: Methods in Molecular Biology ((MIMB,volume 1747))

Abstract

Despite the established importance of nitric oxide (NO) in many physiological and molecular processes in plants, most methods for quantifying NO are open to criticism This reflects the differing methods either lacking specificity or sensitivity, or even from an undue dependence of results on experimental conditions (i.e., chemical concentrations, pH, etc.). In this chapter we describe a protocol to measure gaseous NO produced by a biological sample using quantum cascade laser (QCL)-based spectroscopy. This technique is based on absorption of the laser light by the NO molecules which have been passed from a biological sample into an optical s cell that is equipped with two mirrors placed at both ends. This design greatly increases the interaction path length with the NO molecules due to multiple reflections of the light coupled inside the cell. Thus, the method is able to provide online, in planta measurements of the dynamics of NO production, being highly selective and sensitive (down to ppbv levels;1 ppbv = part per billion by volume mixing ratio = 1:10−9).

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Acknowledgment

This work was supported by the Spanish Ministry of Economy and Competitiveness [AGL2016-78965-R], and the European Regional Development Funds, province of Gelderland [2009-010034]. The work of LM was supported by the BBSRC LINK Grant (BB/I016937/1).

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Authors and Affiliations

  1. Institute for Sustainable Agriculture, Spanish Council for Scientific Research (CSIC), Córdoba, Spain

    Gracia Montilla-Bascón & Elena Prats

  2. Department of Molecular and Laser Physics, Radboud University, Nijmegen, The Netherlands

    Julien Mandon, Frans J. M. Harren & Simona M. Cristescu

  3. Institute of Biological, Environmental and Rural Sciences, University of Aberystwyth, Aberystwyth, UK

    Luis A. J. Mur

Authors
  1. Gracia Montilla-Bascón
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  2. Julien Mandon
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  3. Frans J. M. Harren
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  4. Luis A. J. Mur
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  5. Simona M. Cristescu
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  6. Elena Prats
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Corresponding author

Correspondence to Elena Prats .

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Editors and Affiliations

  1. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Alexander Mengel

  2. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Christian Lindermayr

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Montilla-Bascón, G., Mandon, J., Harren, F.J.M., Mur, L.A.J., Cristescu, S.M., Prats, E. (2018). Quantum Cascade Lasers-Based Detection of Nitric Oxide. In: Mengel, A., Lindermayr, C. (eds) Nitric Oxide. Methods in Molecular Biology, vol 1747. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7695-9_5

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  • DOI: https://doi.org/10.1007/978-1-4939-7695-9_5

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7694-2

  • Online ISBN: 978-1-4939-7695-9

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