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Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Technique

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Nitric Oxide

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1747))

Abstract

Nitric oxide is regarded as a key signaling messenger in several organisms. Its physiological relevance is partly due to its capacity to induce posttranslational modifications of proteins through its direct or indirect reaction with specific amino acid residues. Among them, S-nitrosylation has been shown to be involved in a broad range of cellular signaling pathways both in animals and plants. The identification of S-nitrosylated proteins has been made possible by the development of the Biotin-Switch Technique (BST) in the early 2000s. Here, we describe the BST protocol we routinely use to check in vitro S-nitrosylation of recombinant proteins induced by NO donors.

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Authors and Affiliations

  1. UMR 1347 Agroécologie, AgroSup Dijon, INRA, Univ. Bourgogne-Franche Comté, Dijon Cedex, France

    Sébastien Aimé, Siham Hichami, David Wendehenne & Olivier Lamotte

  2. Pôle Mécanismes et Gestions des Interactions Plantes Microorganismes, CNRS, Dijon Cedex, France

    Sébastien Aimé, Siham Hichami, David Wendehenne & Olivier Lamotte

Authors
  1. Sébastien Aimé
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  2. Siham Hichami
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  3. David Wendehenne
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  4. Olivier Lamotte
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Corresponding author

Correspondence to Olivier Lamotte .

Editor information

Editors and Affiliations

  1. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Alexander Mengel

  2. Helmholtz Zentrum München–German Research GmbH, Center for Environment Health Institute of Biochemical Plant Pathology, Ingolstädter Landstraße 1, Munich-Neuherberg, Germany

    Christian Lindermayr

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Aimé, S., Hichami, S., Wendehenne, D., Lamotte, O. (2018). Analysis of Recombinant Protein S-Nitrosylation Using the Biotin-Switch Technique. In: Mengel, A., Lindermayr, C. (eds) Nitric Oxide. Methods in Molecular Biology, vol 1747. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7695-9_11

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  • DOI: https://doi.org/10.1007/978-1-4939-7695-9_11

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  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-7694-2

  • Online ISBN: 978-1-4939-7695-9

  • eBook Packages: Springer Protocols

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