X-Ray Fluorescence-Detected Flow Cytometry
X-ray fluorescence-detected flow cytometry can enable the detection and characterization of ultra-trace, trace, and bulk elemental content at the cellular level using synchrotron-induced x-ray emission from fully aquated actively respiring cells. Although very much still a technique in development, this technique has been used to characterize cell-to-cell elemental variability in bovine red blood cells, Saccharomyces cerevisiae, and NIH3T3 mouse fibroblasts. Herein we describe the experimental setup and the key methodological aspects of data collection and processing.
KeywordsCytometer Cells Flow cytometer X-ray fluorescence Metallome
This research was funded in part by the National Science Foundation under (NSF-IDBR-0852802 to J.E.P.H.) and used resources of the Advanced Photon Source, a US Department of Energy (DOE) Office of Science User Facility operated for the DOE Office of Science by Argonne National Laboratory under Contract No. DE-AC02-06CH11357.
- 8.O’Malley D (2008) Imaging in depth: controversies and opportunities. In: Correia JJ, Detrich HW (eds) Biophysical tools for biologists, vol 2: In vivo techniques, vol 89. Methods in cell biology. pp 95. doi: https://doi.org/10.1016/s0091-679x(08)00605-5
- 11.Paunesku T, Wanzer MB, Kirillova EN, Muksinova KN, Revina VS, Lyubchansky ER, Grosche B, Birschwilks M, Vogt S, Finney L, Woloschak GE (2012) X-ray fluorescence microscopy for investigation of archival tissues. Health Phys 103(2):181–186. https://doi.org/10.1097/HP.0b013e31824e7023 CrossRefPubMedPubMedCentralGoogle Scholar
- 13.Crawford AM (2015) Methodologies in XRF Cytometry. PhD thesis, University of MichiganGoogle Scholar